Product Information

Contents: Affinity Purified anti-mouse IFN-γ
Catalog Number: 14-7311
Sizes: 50 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: XMG1.2
Isotype: Rat IgG1, κ

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-7311	FITC anti-mouse IFNg (Interferon-gamma, IFN-g)	488	518	IC Flow
12-7311	PE anti-mouse IFNg (Interferon-gamma, IFN-g)	488	575	IC Flow
13-7311	Biotin anti-mouse IFNg (Interferon-gamma, IFN-g)	N/A	N/A	ELISA
14-7311	Affinity Purified anti-mouse IFNg (Interferon-gamma, IFN-g)	N/A	N/A	ELISA cap
16-7311	Functional Grade* Purified anti-mouse IFNg (Interferon-gamma, IFN-g)	N/A	N/A	NU
17-7311	APC anti-mouse IFNg (Interferon-gamma, IFN-g)	633	660	IC Flow
19-7311	Cy5 anti-mouse IFNg (Interferon-gamma, IFN-g)	633	670	IC Flow
25-7311	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse IFNg (Interferon-gamma, IFN-g)	488	760	IC Flow
45-7311	PerCP-Cy5.5 anti-mouse IFNg (Interferon-gamma, IFN-g)	488	690	IC Flow
51-7311	Alexa Fluor® 647 anti-mouse IFNg (Interferon-gamma, IFN-g)	633	668	IC Flow
53-7311	Alexa Fluor® 488 anti-mouse IFNg (Interferon-gamma, IFN-g)	488	519	IC Flow
57-7311	Pacific Blue® anti-mouse IFNg (Interferon-gamma, IFN-g)	402	421	IC Flow
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The XMG1.2 antibody reacts with mouse interferon-γ. The XMG1.2 antibody is a neutralizing antibody. Mouse IFN-γ is a 20 kDa factor produced by activated T, B and NK cells, and is an anti-viral and anti-parasitic cytokine. IFN-γ, in synergy with other cytokines such as TNF-α, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN-γ are exerted on a wide range of cell types expressing the high affinity receptors for IFN-γ. Glycosylation of IFN-γ does not affect its biological activity.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The XMG1.2 antibody has been reported for use in ELISA, intracellular staining for flow cytometric analysis, immunoblotting (WB), and for neutralization of IFN-γ bioactivity.

Applications Tested

The XMG1.2 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse IFNg (Interferon-gamma, IFN-g) in combination with the biotin R4-6A2 (13-7312) antibody for detection and recombinant mouse IFNg (14-8311) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 0.25-1 µg/ml. ***Excess amounts of XMG1.2 may significantly reduce ELISA sensitivity.*** A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/ml - 15 pg/ml should be included in each ELISA plate.

References

Abrams, J. S., M. G. Roncarolo, et al. (1992). "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." Immunol Rev 127: 5-24.

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Cat. 13-7312    Biotin anti-mouse IFNg (Interferon-gamma, IFN-g) (clone R4-6A2)
Cat. 14-8311    Recombinant Mouse IFNg (Interferon-gamma, IFN-g)