Product Information

Contents: Affinity Purified anti-rat IFN-γ
Catalog Number: 14-7310
Sizes: 50 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: DB-1 (DB1)
Isotype: Mouse IgG1, κ

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-7310	Affinity Purified anti-rat IFNg (Interferon-gamma, IFN-g)	N/A	N/A	ELISA cap

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The DB-1 monoclonal antibody reacts with rat interferon-gamma (IFN-γ), a 15-20 kDa cytokine secreted by Th1 cells. For optimal detection of rat IFN-γ by sandwich ELISA, a combination of purified DB-1 for capture and biotinylated polyclonal anti-rat IFN-γ for detection is recommended. DB-1 is used for capture of rat IFN-γ by ELISA.

Applications Reported

For research use only, not for diagnostic or therapeutic use. DB-1 has been reported for use in capture of rat IFN-γ by ELISA.

Applications Tested

The DB-1 (DB1) antibody has been tested as the capture antibody in a sandwich ELISA for analysis of rat IFNg (Interferon-gamma, IFN-g) in combination with the biotin anti-rat IFNg poly (13-7320) antibody for detection and recombinant rat IFNg (14-8315) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/ml - 15 pg/ml should be included in each ELISA plate.

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