Mouse IL-27 Recombinant Protein (cat. 14-8271) (lane 1) and mouse bone marrow-derived dendritic cells stimulated with LPS (lane 2) or left untreated (lane 3) were analyzed by SDS-PAGE followed by immunoblotting with 5 µg/ml of Anti-Mouse EBI3 (IL-27 subunit) Purified. Bands were visualized with Anti-Rat IgG HRP (cat. 18-4818).
Contents: Anti-Mouse EBI3 (IL-27 subunit) Purified Catalog Number: 14-7273 Concentration: 0.5 mg/ml Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary Storage Conditions: Store at 2-8°C. Clone: DNT27 Host/Isotype: Rat IgG2a, k
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14-7273-80
25 ug
14-7273-82
100 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The monoclonal antibody DNT27 reacts with mouse Epstein-Barr Virus-induced gene 3 (EBI3). EBI3 is a 34 kDa soluble protein first identified in B cells that is upregulated after Epstein-Barr virus infection. EBI3 can exist as a homodimer and also heterodimerizes with IL-27α (p28, IL-30) to form the cytokine IL-27. IL-27 is produced most abundantly by dendritic cells and macrophages with some recent reports of expression in NKT cells. Recently, a complex of EBI3 and the p35 subunit of IL-12, called IL-35, has been reported to be produced by regulatory T cells.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This DNT27 antibody has been reported for use in immunoblotting (WB).
Applications Tested
This DNT27 antibody has been tested by western blot of cell lysates prepared from mouse EBI3-transfected 293T cells and bone marrow-derived dendritic cells. This can be used at less than or equal to 1-5 μg/ml. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Fujita H, Teng A, Nozawa R, Takamoto-Matsui Y, Katagiri-Matsumura H, Ikezawa Z, Ishii Y. Production of both IL-27 and IFN-{gamma} after the treatment with a ligand for invariant NK T cells is responsible for the suppression of Th2 response and allergic inflammation in a mouse experimental asthma model. J Immunol. 2009 Jul 1;183(1):254-60.
Collison LW, Vignali DA. Interleukin-35: odd one out or part of the family? Immunol Rev. 2008 Dec;226:248-62.
Stumhofer JS, Hunter CA. Advances in understanding the anti-inflammatory properties of IL-27. Immunol Lett. 2008 May 15;117(2):123-30.
Pflanz S, Timans JC, Cheung J, Rosales R, Kanzler H, Gilbert J, Hibbert L, Churakova T, Travis M, Vaisberg E, Blumenschein WM, Mattson JD, Wagner JL, To W, Zurawski S, McClanahan TK, Gorman DM, Bazan JF, de Waal Malefyt R, Rennick D, Kastelein RA.
IL-27, a heterodimeric cytokine composed of EBI3 and p28 protein, induces proliferation of naïve CD4(+) T cells. Immunity. 2002 Jun;16(6):779-90.
Devergne O, Coulomb-L'Herminé A, Capel F, Moussa M, Capron F. Expression of Epstein-Barr virus-induced gene 3, an interleukin-12 p40-related molecule, throughout human pregnancy: involvement of syncytiotrophoblasts and extravillous trophoblasts. Am J Pathol. 2001 Nov;159(5):1763-76.
