Product Information

Contents: Affinity Purified anti-human IL-18 Receptor α chain (CDw218a, IL-1Rrp)
Catalog Number: 14-7183
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: H44
Isotype: Mouse IgG1, κ

Surface staining of normal human peripheral blood cells with anti-human IL-18Ra (Interleukin-18 Receptor alpha, IL-18 Receptor alpha chain, IL-1Rrp) (H44) PE. Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-7183	Affinity Purified anti-human IL-18Ra (CD218a, Interleukin-18 Receptor alpha, IL-18 Receptor alpha chain, IL-1Rrp, IL18R)	N/A	N/A	FC
13-7183	Biotin anti-human IL-18Ra (CD218a, Interleukin-18 Receptor alpha, IL-18 Receptor alpha chain, IL-1Rrp, IL18R)	N/A	N/A	FC
11-7183	FITC anti-human IL-18Ra (CD218a, Interleukin-18 Receptor alpha, IL-18 Receptor alpha chain, IL-1Rrp, IL18R)	488	518	FC
12-7183	PE anti-human IL-18Ra (CD218a, Interleukin-18 Receptor alpha, IL-18 Receptor alpha chain, IL-1Rrp, IL18R)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The H44 antibody reacts with the interleukin-18 receptor α chain (IL-18Rα), also known as IL-1Rrp, an IL-1R family member. IL-18Rα is expressed on Th1 cells, NK cell subset, neutrophils and IL-12-stimulated tonsillar B cells. By itself, IL-18Rα binds IL-18 with low affinity and when complexed with IL-18Rβ chain binds IL-18 with high affinity. The proinflammatory cytokine IL-18 and its receptor complex play an important role in the innate and autoimmune responses.

Applications Reported

For research use only, not for diagnostic or therapeutic use. H44 has been reported for use in flow cytometric analysis, immunohistochemistry, immunoprecipitation, immunoblotting (WB), and in vitro neutralization. (Please use Functional Grade purified cat.16-7183 for functional studies).

Applications Tested

This H44 antibody has been tested by flow cytometric analysis on peripheral blood leukocytes and granulocytes. This can be used at less than or equal to 0.5 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Tominaga, K., T. Yoshimoto, et al. 2000. IL-12 synergizes with IL-18 or IL-1 beta for INF-gamma production from human T cells. Int. Immunol. 12:151
Chan, W. L., N. Pejnovic, et al. 2001. Human IL-18 receptor and ST2L are stable and selective markers for the respective type 1 and type 2 circulating lymphocytes. J. Immunol. 167:1238
Leung BP, Culshaw S, et al. 2001. A role for IL-18 in neutrophil activation. J Immunol 2001. 167:2879
Gerdes N, Sukhova GK, et al. 2002. Expression of interleukin (IL)-18 and functional IL-18 receptor on human vascular endothelial cells, smooth muscle cells, and macrophages: implications for atherogenesis. J Exp Med. 195:245
Fujimori Y, Yoshimoto T, et al. 2002. Increased expression of interleukin-18 receptor on T lymphocytes in patients with acute graft-versus-host disease after allogeneic bone marrow transplantation. J Interferon Cytokine Res 22:751
Nakahira M, Tomura M, et al. 2001. An absolute requirement for STAT4 and a role for IFN-gamma as an amplifying factor in IL-12 induction of the functional IL-18 receptor complex. J Immunol. 167:1306

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