14-7172

Contents: Affinity Purified anti-mouse IL-17A (Interleukin-17A)
Catalog Number: 14-7172
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBioTC11-18H10.1
Isotype: Rat IgG1, κ

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
51-7172	Alexa Fluor® 647 anti-mouse IL-17A (Interleukin-17A, IL17A)	633	668	IC Flow
53-7172	Alexa Fluor® 488 anti-mouse IL-17A (Interleukin-17A, IL17A)	488	519	IC Flow

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eBioTC11-18H10.1 antibody reacts with mouse IL-17A. The eBioTC11-18H10.1 antibody is a neutralizing antibody. Interleukin-17A (IL-17A) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. The cDNA encoding human IL-17A was isolated from a library of CD4+ T cells; the encoded protein exhibits 72 percent amino acid identity with HVS13 , an open reading frame from a T lymphotropic Herpesvirus saimiri, and 63 percent with mouse CTLA-8 (cytotoxic T-lymphocyte associated antigen-8). Human IL-17A exists as glycosylated 20-30 kD homodimers. High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells. IL-17A enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. Human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse IL-17A receptor.

IL-23-dependent, IL-17A-producing CD4+ T cells (Th-17 cells) have been identified as a unique subset of Th cells that develops along a pathway that is distinct from the Th1- and Th2- cell differentiation pathways. The hallmark effector molecules of Th1 and Th2 cells, e.g., IFN-γ and IL-4, have each been found to negatively regulate the generation of these Th-17 cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The eBioTC11-18H10.1 antibody has been reported for use as the capture antibody in mouse IL-17A ELISA and ELISPOT assay, for neutralization of IL-17A bioactivity, and for intracellular staining of IL-17A-producing cells.

Applications Tested

The Affinity Purified eBioTC11-18H10.1 antibody has been tested as the capture antibody in a sandwich ELISA for measurement of mouse IL-17A protein levels, in combination with the biotinylated eBioTC11-8H4 antibody (13-7171) for detection and recombinant mouse IL-17A (14-8171) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 0.5 – 2.0 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/ml - 15 pg/ml should be included in each ELISA plate.

The Functional Grade Purified eBioTC11-18H10.1 antibody has been tested for neutralization of IL-17A bioactivity and for ELISPOT capture.

The fluorochrome-conjugated eBioTC11-18H10.1 antibody has been tested by intracellular staining for flow cytometric analysis and can be used at less than or equal to 0.50 µg/million cells/100 ul staining volume.

References

Mangen PR, Harrington LE, O'Quinn DB, Helms WS, Bullard DC, Elson CO, Hatton RD, Wahl SM, Schoeb TR, Weaver CT. 2006. Transforming growth factor-beta induces development of the Th-17 lineage. Nature. Letters. Advance Online Publication. 30 April, 2006. [Intracellular staining]
Harrington LE, Hatton RD, Mangan PR, Turner H, Murphy TL, Murphy KM, Weaver CT. 2005. Interleukin 17–producing CD4+ effector T cells develop via a lineage distinct from the T helper type 1 and 2 lineages. Nature Immunol 6: 1123-1132. [ELISA, Intracellular Staining]
Park H, Li X, Yang XO, Chang SH, Nurieva R, Wang Y-H, Wang Y, Hood L, Zhu S, Tian Q, Dong C. 2005. A distinct lineage of CD4 T cells regulates tissue inflammation by producing interleukin 17. Nature Immunol 6: 1133-1141. [ELISA, Intracellular Staining]
Nekrasova T, Shive C, Gao Y, Kawamura K, Guardia R, Landreth G, Forsthuber TG. 2005. ERK1-deficient mice show normal T cell effector function and are highly susceptible to experimental autoimmune encephalomyelitis. J Immunol. 175: 2374-80. [ELISPOT]
Schubert D, Maier B, Morawietz L, Krenn V, Kamradt T. 2004. Immunization with Glucose-6-Phosphate Isomerase Induces T Cell-Dependent Peripheral Polyarthritis in Genetically Unaltered Mice. J Immunol 172: 4503-4509. [Intracellular Staining]
Infante-Duarte C, Horton HF, Byrne MC, Kamradt T. 2000. Microbial Lipopeptides Induce the Production of IL-17 in Th Cells. J Immunol. 165: 6107-6115. [Intracellular Staining]
Hofstetter HH, Ibraihim SM, Koczan D, Kruse N, Weishaupt A, Toyka KV, Gold R. 2005. Therapeutic efficacy of IL-17 neutralization in murine experimental autoimmune encephalomyelitis. Cell Immunol. [Dec 27 2005 Epub ahead of print; ELISPOT]

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