Product Information

Contents: Affinity Purified anti-human IL-15R (Interleukin-15 Receptor, IL-15 Receptor)
Catalog Number: 14-7159
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBioJM7A4
Isotype: Mouse IgG2b

Normal human PBMCs were isolated, and cultured in standard conditions for 3 hours. Non-adherent cells were removed from culture, and remaining cells were stimulated with LPS and IFN-γ for 24 hours.

The figure demonstrates staining of normal human PBMCs with 0.25 μg of Purified Mouse IgG2b Iso Cntrl (cat. 14-4732) (open histogram) or 0.25 μg of Purified anti-human IL-15R (colored histogram) followed by Biotin Anti-Mouse IgG (cat. 13-4013)and SAv-PE (cat. 12-4317). Cells in the large scatter population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-7159	Fluorescein isothiocyanate (FITC) anti-human IL-15Ra (Interleukin-15 Receptor Alpha, IL-15 Receptor)	488	518	FC
12-7159	Phycoerythrin (PE) anti-human IL-15Ra (Interleukin-15 Receptor Alpha, IL-15 Receptor)	488	575	FC
14-7159	Affinity Purified anti-human IL-15Ra (Interleukin-15 Receptor Alpha, IL-15 Receptor)	N/A	N/A	FC IP WB

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eBioJM7A4 monoclonal antibody reacts with the IL-15 receptor alpha chain (IL-15Rα). IL-15 and IL-2 are cytokines with overlapping, but distinct, biologic effects. Their receptors share 2 subunits, the IL2R-β and -γ chains, which are essential for signal transduction. The IL-15R-α receptor is structurally related to IL-2R-α. However, the IL-15Rα alone binds IL-15 with a 1,000-fold higher affinity than that seen with IL-2R-α and IL-2. In the human, 3 differentially spliced human IL15R-α variants that are all capable of high affinity binding of IL-15 have been isolated. The cytoplasmic domain of IL15R-α, like that of IL2R-α, is dispensable for mitogenic signaling, suggesting that the primary role of the alpha chains is to confer high affinity binding. At high concentrations, IL-15, like IL-2, is able to signal through a complex of IL2R-β and IL2R-γ in the absence of the alpha subunit.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioJM7A4 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunoblotting (WB).

Applications Tested

This eBioJM7A4 antibody has been tested by flow cytometric analysis of LPS and IFN-γ-stimulated PBMCs. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Vamosi G, Bodnar A, Vereb G, Jenei A, Goldman CK, Langowski J, Toth K, Matyus L, Szollosi J, Waldmann TA, Damjanovich S. IL-2 and IL-15 receptor alpha-subunits are coexpressed in a supramolecular receptor cluster in lipid rafts of T cells. Proc Natl Acad Sci U S A. 2004 Jul 27;101(30):11082-7. (JM7A4, FRET, PubMed)

Dubois S, Mariner J, Waldmann TA, Tagaya Y. IL-15Ralpha recycles and presents IL-15 In trans to neighboring cells. Immunity. 2002 Nov;17(5):537-47. (JM7A4, FC, WB, PubMed)

Anderson, D., et al. 1995. Functional characterization of the human interleukin-15 receptor alpha chain and close linkage of IL15RA and IL2RA genes. J. Biol. Chem. 270: 29862-29869.

Giri, J., et al. 1995. Identification and cloning of a novel IL-15 binding protein that is structurally related to the alpha of the IL-2 receptor. EMBO J. 14: 3654-3663.

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