Product Information

Contents: Affinity Purified anti-human interleukin (IL-13)
Catalog Number: 14-7139
Sizes: 50 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: PVM13-1
Isotype: Mouse IgG1

Human PBMCs (300,000/well) in 100 ul complete RPMI medium were treated without (left) or with (right) Candida (100ug/mL) for 24 hrs in an IL-13 ELISPOT assay plate (pre-coated with functional grade PVM13-1 antibody).

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-7139	Fluorescein (FITC) anti-human IL-13 (Interleukin-13, IL13)	488	518	IC Flow
14-7139	Affinity Purified anti-human IL-13 (Interleukin-13, IL13)	N/A	N/A	ELISA cap ELISPOT IC Flow NU
16-7139	Functional Grade* Purified anti-human IL-13 (Interleukin-13, IL13)	N/A	N/A	ELISPOT cap NU
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The PVM13-1 antibody reacts with human interleukin-13 (IL-13). IL-13, also known as NC30, is a 12.5 kDa protein secreted by activated T helper cells, CD8⁺ T cells, and NK cells. The biological activities of IL-13 include suppression of macrophage cytotoxic activity, upregulation of IL-1RA expression and suppression of inflammatory cytokine expression.

Applications Reported

For research use only, not for diagnostic or therapeutic use. PVM13-1 has been reported for use in intracellular flow cytometric analysis, cytokine neutralization, and ELISA. (Fluorochrome conjugated PVM13-1 is recommended for use in intracellular flow cytometry.) (Please use Functional Grade purified PVM13-1, cat. 16-7139, in functional assays.)

Applications Tested

The PVM13-1 antibody has been tested as the capture antibody in a sandwich ELISA and ELISPOT assay for analysis of human interleukin-13 (IL-13) in combination with the biotinylated anti-human IL-13 polyclonal antibody (13-7138) antibody for detection and recombinant human IL-13 (14-8139) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/ml - 8 pg/ml should be included in each ELISA plate.

References

Griffioen, M., et al. 2004. Cancer Immunol. Immunother. 53: 715-722.
Van Halteren, A.G., et al. 2002. Diabetes 51: 2119-2125.
Martin, S., et al. 2001. N. Engl. J. Med. 345: 1036-1040.
Raz, I., et al.. 2001. Lancet 358: 1749-1753.
Schloot, N., et al. 2003. J. Autoimmun. 21: 365-376.

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