14-7135

Contents: Affinity Purified anti-mouse IL-13 (Interleukin-13)
Catalog Number: 14-7135
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBio1316H
Isotype: Rat IgG1, κ

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
16-7135	Functional Grade* Purified anti-mouse IL-13 (Interleukin-13, IL13)	N/A	N/A	NU
13-7135	Biotin anti-mouse IL-13 (Interleukin-13, IL13)	N/A	N/A	ELISA det ELISPOT det
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eBio1316H antibody reacts with mouse IL-13. The eBio1316H antibody is a neutralizing antibody. Mouse interleukin-13 (IL-13), originally designated p600, is a 11.6 kDa protein secreted by activated T helper cells, CD8+ T cells, and NK cells. IL-13 was originally identified in 1989 through differential hybridization of cDNA libraries prepared from T helper 1 and T helper 2 cells. IL-13 contains four cysteine residues, which form two intramolecular disulfide bonds. IL-13 shares many activities with IL-4; this relates to a requirement for IL-4Ralpha chain for signal transduction. The biological activities of IL-13 include suppression of macrophage cytotoxic activity, upregulation of IL-1RA expression, and suppression of inflammatory cytokine expression. IL-13 induces CD23 expression on B cells and enhances CD72, and expression of class II MHC gene products. Mouse IL-13 does not display the activities on T cells and B cell stimulatory effects which IL-4 exhibits. Mouse IL-13 is active on human and mouse cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use.

Applications Tested

The eBio1316H antibody has been tested as the capture antibody in a sandwich ELISA (and ELISPOT) for analysis of mouse IL-13, in combination with the biotinylated eBio13A antibody for detection (13-7133) and recombinant mouse IL-13 (14-8131) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1.0 - 4.0 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/ml - 8.0 pg/ml should be included in each ELISA plate.
The Functional Grade Purified eBio1316H antibody (16-7135) is recommended for in vitro blocking studies.

Related Products

Cat. 14-4301    Affinity Purified Rat IgG1 Isotype Control
Cat. 14-7131    Affinity Purified anti-mouse IL-13 (Interleukin-13, IL13) (clone eBioMTZ4513)
Cat. 14-7139    Affinity Purified anti-human IL-13 (Interleukin-13, IL13) (clone PVM13-1)
Cat. 14-8131    Recombinant Mouse IL-13 (Interleukin-13, IL13)
Cat. 14-8139    Recombinant Human IL-13 (Interleukin-13, IL13)