Product Information

Contents: Affinity Purified anti-mouse IL-13 (Interleukin-13)
Catalog Number: 14-7131
Sizes: 25 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBioMTZ4513
Isotype: Rat IgG2b

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-7131	DISCONTINUED - Affinity Purified anti-mouse IL-13 (Interleukin-13, IL13)	N/A	N/A	ELISA cap

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eBioMTZ4513 antibody reacts with mouse IL-13. The eBioMTZ4513 antibody is useful as the capture antibody for a mouse IL-13 sandwich ELISA.

Mouse interleukin-13 (IL-13), originally designated p600, is a 11.6 kDa protein secreted by activated T helper cells, CD8+ T cells, and NK cells. IL-13 was originally identified in 1989 through differential hybridization of cDNA libraries prepared from T helper 1 and T helper 2 cells. IL-13 contains four cysteine residues, which form two intramolecular disulfide bonds. IL-13 shares many activities with IL-4; this relates to a requirement for IL-4Rα chain for signal transduction. The biological activities of IL-13 include suppression of macrophage cytotoxic activity, upregulation of IL-1RA expression, and suppression of inflammatory cytokine expression. IL-13 induces CD23 expression on B cells and enhances CD72, and expression of class II MHC gene products. Mouse IL-13 does not display the activities on T cells and B cell stimulatory effects which IL-4 exhibits. Mouse IL-13 is active on human and mouse cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The eBioMTZ4513 antibody has been reported for use as the capture antibody in a mouse IL-13 ELISA.

Applications Tested

The eBioMTZ4513 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse IL-13, in combination with the biotinylated F(ab)'2 of an antigen-affinity purified polyclonal rabbit anti-mouse IL-13 antibody for detection and recombinant mouse IL-13 (14-8131) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 2-6 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 4000 pg/ml - 30 pg/ml should be included in each ELISA plate.

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