Product Information

Contents: Affinity Purified anti-human interleukin-12 (IL-12)
Catalog Number: 14-7128
Sizes: 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: B-T21 (BT21)
Isotype: Mouse IgG1

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-7128	Affinity Purified anti-human IL-12 (Interleukin-12, IL12)	N/A	N/A	ELISA cap

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Description

The B-T21 antibody reacts with the p35 subunit of human interleukin-12 (IL-12). IL-12 is a 70 kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and is secreted primarily by activated B cells. For optimal detection of human IL-12 p70 by ELISA, a combination of purified B-T21 antibody for capture and biotinylated C8.6 antibody for detection is recommended.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The B-T21 antibody has been reported for use as capture antibody in an IL-12 p70 sandwich ELISA.

Applications Tested

The B-T21 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human IL-12 p70 in combination with the biotinylated C8.6 (13-7129) antibody for detection and recombinant human IL-12 p70 (14-8129) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1 - 4 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/ml - 15 pg/ml should be included in each ELISA plate.

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