Product Information

Contents: Affinity Purified anti-mouse interleukin-12 (IL-12) p40/p70
Catalog Number: 14-7125
Sizes: 50 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: C15.6
Isotype: Rat IgG1

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-7125	Affinity Purified anti-mouse IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40	N/A	N/A	ELISA cap

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The C15.6 antibody reacts with the p40 subunit of mouse interleukin-12 and IL-23. IL-12 is a 70 kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and is secreted by activated B cells and macrophages. IL-23 is a structurally and biologically-related cytokine comprised of the same p40 subunit and a p19 subunit. For optimal detection of total IL-12 p40 (monomer, heterodimer, homodimer) by ELISA, a combination of purified C15.6 antibody for capture and biotinylated C17.8 antibody for detection is recommended. Fluorochrome conjugated C15.6 antibody is effective for staining intracellular IL-12.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The C15.6 antibody has been reported for use in ELISA.

Applications Tested

The C15.6 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse IL-12 (p40 monomer or p40 dimerized with p35) or mouse IL-23 (p40 dimerized with p19) in combination with the biotin C17.8 (13-7123) antibody for detection and recombinant mouse IL-12 p70 (14-8121) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 0.25-1 µg/ml. A standard curve consisting of doubling dilutions of the recombinant IL-12 p70 standard over the range of 2000 pg/ml - 15 pg/ml should be included in each ELISA plate.

References

Wysocka, M., et al. (1995). Interleukin-12 is required for interferon-gamma production and lethality in lipopolysaccharide-induced shock in mice. Eur. J. Immunol. 25: 671-676.

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