mouse IL-12 antibody, Affinity Purified conjugate

Contents: Affinity Purified anti-mouse interleukin-12 (IL-12) p40/p70
Catalog Number: 14-7125
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: C15.6
Isotype: Rat IgG1

Product Options

Price

Cat. No.

Size

To view relevant pricing,
please select your country
and resume browsing.

14-7125-81

50 ug

14-7125-85

500 ug

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-7125	Affinity Purified anti-mouse IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40	N/A	N/A	ELISA cap

Custom Product Requests
Need a custom product? Download the Custom Product Request Form and submit completed form to customs@ebioscience.com.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The C15.6 antibody reacts with the p40 subunit of mouse interleukin-12 and IL-23. IL-12 is a 70 kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and is secreted by activated B cells and macrophages. IL-23 is a structurally and biologically-related cytokine comprised of the same p40 subunit and a p19 subunit. For optimal detection of total IL-12 p40 (monomer, heterodimer, homodimer) by ELISA, a combination of purified C15.6 antibody for capture and biotinylated C17.8 antibody for detection is recommended. Fluorochrome conjugated C15.6 antibody is effective for staining intracellular IL-12.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The C15.6 antibody has been reported for use in ELISA.

Applications Tested

The C15.6 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse IL-12 (p40 monomer or p40 dimerized with p35) or mouse IL-23 (p40 dimerized with p19) in combination with the biotin C17.8 (13-7123) antibody for detection and recombinant mouse IL-12 p70 (14-8121) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 0.25-1 µg/ml. A standard curve consisting of doubling dilutions of the recombinant IL-12 p70 standard over the range of 2000 pg/ml - 15 pg/ml should be included in each ELISA plate.

References

Wysocka, M., et al. (1995). Interleukin-12 is required for interferon-gamma production and lethality in lipopolysaccharide-induced shock in mice. Eur. J. Immunol. 25: 671-676.

Related Products

Cat. 00-0000  eBioscience 10X ELISA Coating Buffer
Cat. 44-2404  Nunc MaxiSorp™ flat-bottom 96 well plate
Cat. 18-4100  Avidin Horse Radish Peroxidase (AV-HRP)
Cat. 00-4202  eBioscience ELISA Diluent Solution, 5X
Cat. 00-4203  Super AquaBlue ELISA Substrate
Cat. 14-4301  Affinity Purified Rat IgG1 Isotype Control
Cat. 88-7121  Mouse IL-12 (Interleukin-12, IL12) p70 ELISA Ready-SET-Go!
Cat. 88-7231  Mouse IL-23 (Interleukin-23, IL23) ELISA Ready-SET-Go! Kit (with Pre-Coated Plates)
Cat. 88-7234  Mouse IL-23 (p19/p40, IL23) ELISA Ready-SET-Go!
Cat. 88-7921  Mouse IL-12 (Interleukin-12, IL12) p70 ELISA Ready-SET-Go! Kit (with Pre-Coated Plates)
Cat. 14-8121  Recombinant Mouse IL-12 (Interleukin-12, IL12) p70
Cat. 34-8231  Carrier-Free Recombinant Mouse IL-23 (Interleukin-23, IL23)