Product Information

Contents: Affinity Purified anti-mouse interleukin-12 (IL-12) p35/p70
Catalog Number: 14-7122
Sizes: 50 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: C18.2
Isotype: Rat IgG2a, κ

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-7122	Affinity Purified anti-mouse IL-12 (Interleukin-12, IL12)	N/A	N/A	ELISA cap

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The C18.2 antibody reacts with the p35 subunit of mouse interleukin-12 p70 (IL-12 p70). The C18.2 antibody is a non-neutralizing antibody. Interleukin-12 (IL-12) is a heterodimeric 70 kD (p70) cytokine composed of two covalently linked, glycosylated chains, 40kD (p40) and 35-kD (p35). IL-12 is mainly produced by monocytes, macrophages, and dendritic cells in response to bacterial products such as lipopolysaccharides (LPS), to intracellular pathogens or upon interaction with activated T cells. IL-12 was originally discovered because of its ability to induce interferon-gamma (IFN-g) production, cell proliferation, and cytotoxicity mediated by natural killer cells and T cells. It is now established that IL-12 also plays a key role in the development of Th1 responses, leading to IFN-g and IL-2 production. These cytokines can in turn promote T-cell responses and macrophage activation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The C18.2 antibody is reported for use as the capture antibody in a mouse IL-12 p70 ELISA.

Applications Tested

The C18.2 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of bioactive mouse IL-12 p70 in combination with the biotinylated C17.8 (13-7123) antibody for detection and recombinant mouse IL-12 p70 (14-8121) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/ml - 15 pg/ml should be included in each ELISA plate.
The C18.2 antibody is a non-neutralizing antibody.

References

Cho D, Lee WJ, Halloran PJ, Trinchieri G, Kim YB. Enhancement of porcine natural killer cell activity by recombinant human and murine IL-12. Cell Immunol. 1996 Aug 25;172(1):29-34.
Aragane Y, Riemann H, Bhardwaj RS, Schwarz A, Sawada Y, Yamada H, Luger TA, Kubin M, Trinchieri G, Schwarz T. IL-12 is expressed and released by human keratinocytes and epidermoid carcinoma cell lines. J Immunol. 1994 Dec 15;153(12):5366-72.
Wysocka M, Kubin M, Vieira LQ, Ozmen L, Garotta G, Scott P, Trinchieri G. Interleukin-12 is required for interferon-gamma production and lethality in lipopolysaccharide-induced shock in mice.
Eur J Immunol. 1995 Mar;25(3):672-6.
Wysocka M, Robertson S, Riemann H, Caamano J, Hunter C, Mackiewicz A, Montaner LJ, Trinchieri G, Karp CL.
IL-12 suppression during experimental endotoxin tolerance: dendritic cell loss and macrophage hyporesponsiveness.
J Immunol. 2001 Jun 15;166(12):7504-13.

Related Products

Cat. 14-4321    Affinity Purified Rat IgG2a Isotype Control
Cat. 12-7123    PE anti-mouse IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40 (clone C17.8)
Cat. 13-7123    Biotin anti-mouse IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40 (clone C17.8)
Cat. 16-7123    Functional Grade Purified anti-mouse IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40 (clone C17.8)
Cat. 88-7231    Mouse IL-23 (Interleukin-23, IL23) ELISA Ready-SET-Go! Kit (with Pre-Coated Plates)
Cat. 16-7232    Functional Grade Purified anti-mouse IL-23 (Interlekin-23, IL23) p19 (clone G23-8)
Cat. 88-7234    Mouse IL-23 (p19/p40, IL23) ELISA Ready-SET-Go!
Cat. 88-7921    Mouse IL-12 (Interleukin-12, IL12) p70 ELISA Ready-SET-Go! Kit (with Pre-Coated Plates)
Cat. 14-8231    Recombinant Mouse IL-23 (Interleukin-23, IL23)
Cat. 34-8231    Carrier-Free Recombinant Mouse IL-23 (Interleukin-23, IL23)