human IL-10 (Interleukin-10) antibody: purified antibody

Contents: Affinity Purified anti-human interleukin-10 (IL-10)
Catalog Number: 14-7108
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: JES3-9D7
Isotype: Rat IgG1, κ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
14-7108-81	50 ug	$80
14-7108-85	500 ug	$220

*International customers: Please contact your distributor for region specific pricing.

Human peripheral blood mononuclear cells were stimulated with PMA and ionomycin in the presence of Brefeldin A for 5 hours. The cells were surface stained with FITC anti-human CD4 (RPA-T4) and intracellularly stained with PE anti-human IL-10 (JES3-9D7).

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-7108	PE anti-human IL-10 (Interleukin-10, IL10)	488	575	IC Flow
14-7108	Affinity Purified anti-human IL-10 (Interleukin-10, IL10)	N/A	N/A	ELISA cap ELISPOT cap IHC(Paraffin)
16-7108	Functional Grade* Purified anti-human IL-10 (Interleukin-10, IL10)	N/A	N/A	ELISPOT cap NU
51-7108	Alexa Fluor® 647 anti-human IL-10 (Interleukin-10, IL10)	633	668	IC Flow
53-7108	Alexa Fluor® 488 anti-human IL-10 (Interleukin-10, IL10)	488	519	IC Flow
57-7108	Pacific Blue® anti-human IL-10 (Interleukin-10, IL10)	402	421	IC Flow
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The JES3-9D7 monoclonal antibody reacts with human interleukin-10 (IL-10).

Applications Reported

For research use only, not for diagnostic or therapeutic use. The JES3-9D7 antibody has been reported for use in capture of human IL-10 by ELISA, intracellular flow cytometric analysis, and for neutralizing IL-10 bioactivity. (Use Functional Grade purified for functional studies cat.16-7108).

Applications Tested

The JES3-9D7 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human Interleukin-10 (IL-10) in combination with the biotin JES3-12G8 (13-7109) antibody for detection and recombinant human IL-10 (14-8109) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/ml - 8 pg/ml should be included in each ELISA plate.

References

Abrams, J. S., M. G. Roncarolo, et al. 1992. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev 127: 5-24.

Chandler SW, Rassekh CH, et al. 2002. Immunohistochemical localization of interleukin-10 in human oral and pharyngeal carcinomas. Laryngoscope. 112(5):808-15. (IHC paraffin, PubMed)

Sendide K, Deghmane AE, et al. 2005. Mycobacterium bovis BCG attenuates surface expression of mature class II molecules through IL-10-dependent inhibition of cathepsin S. J Immunol. 175(8):5324-32. (FA, PubMed)

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