14-7102

Contents: Affinity Purified anti-mouse interleukin-10 (IL-10)
Catalog Number: 14-7102
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: JES5-2A5
Isotype: Rat IgG1, κ

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
13-7102	Biotin anti-mouse IL-10 (Interleukin-10, IL10)	N/A	N/A	ELISA det ELISPOT det
16-7102	Functional Grade* Purified anti-mouse IL-10 (Interleukin-10, IL10)	N/A	N/A	NU
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The JES5-2A5 antibody reacts with mouse interleukin-10 (IL-10). The JES5-2A5 antibody is a neutralizing antibody. Mouse IL-10 is an 18 kDa factor also known as Cytokine Synthesis Inhibitory Factor (CSIF). In the mouse, Th2 cells, B1 cells, macrophages, and keratinocytes are the major cell subsets that produce IL-10. IL-10 inhibits synthesis of Th1 cytokines and proliferation of T cells, and acts as a costimulatory signal for mast cells, developing thymocytes and the Th2 response.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The JES5-2A5 antibody has been reported for use in ELISA, ELISPOT, neutralization of IL-10 bioactivity, and intracellular staining for flow cytometric analysis.

Applications Tested

The Functional Grade Purified JES5-2A5 antibody has been tested by LAL assay to verify low endotoxin levels, by ELISA, and in bioassay for neutralization of mouse IL-10 bioactivity.

The JES5-2A5 antibody at 0.004 ug/ml has been found to inhibit by 50% the biological effects of 1.0 ng/ml mouse IL-10 in an MC/9 cell proliferation assay. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols section.

The biotinylated JES5-2A5 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse interleukin-10 (IL-10) in combination with the purified JES5-16E3 (14-7101) antibody for capture and recombinant mouse IL-10 (14-8101) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 4000 pg/ml - 30 pg/ml should be included in each ELISA plate.

References

Abrams, J. S., M. G. Roncarolo, et al. (1992). "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." Immunol Rev 127: 5-24.
Sander, B., I. Hoiden, et al. (1993). "Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining." J Immunol Methods 166(2): 201-14.

Related Products

Cat. 13-7101    Biotin anti-mouse IL-10 (Interleukin-10, IL10) (clone JES5-16E3)
Cat. 88-7134    Mouse IL-13 (Interleukin-13, IL13) ELISA Ready-SET-Go!
Cat. 88-7150    Mouse IL-15 (Interleukin-15, IL15) ELISA Ready-SET-Go!
Cat. 88-7174    Mouse IL-17A (Interleukin-17A, IL17A) ELISA Ready-SET-Go!
Cat. 88-7224    **Please Inquire** Mouse LAG-3 (sCD223) ELISA Ready-SET-Go!
Cat. 88-7234    Mouse IL-23 (p19/p40, IL23) ELISA Ready-SET-Go!
Cat. 88-7274    Mouse IL-27 (p28/EBI3, IL27) ELISA Ready-SET-Go!
Cat. 34-8041    Carrier-Free Recombinant Mouse IL-4 (Interleukin-4, IL4)
Cat. 34-8051    Carrier-Free Recombinant Mouse IL-5 (Interleukin-5, IL5)
Cat. 34-8101    Carrier-Free Recombinant Mouse IL-10 (Interleukin-10, IL10)
Cat. 34-8171    Carrier-Free Recombinant Mouse IL-17A (Interleukin-17A, IL17A)