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Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq. DescriptionThe 5D3-F7 antibody reacts with human, cynomolgus, and rhesus monkey monocyte chemoattractant protein-1 (MCP-1), also known as monocyte chemotactic and activating factor (MCAF) and CCL2. MCP-1 is a CC chemokine which presents as a dimer. Derived from monocytes and vascular endothelial cells, it exerts chemoattractant activity on a variety of cell types, such as monocytes, basophils, T cells and NK cells via chemokine receptors such as CCR2, CCR5, and CCR10. Applications ReportedFor research use only, not for diagnostic or therapeutic use. The 5D3-F7 antibody has been reported to be useful for detection of MCP-1 in a sandwich ELISA, neutralization of MCP-1 bioactivity, intracellular staining for flow cytometric analysis, IP, and immunohistochemical staining of frozen tissue sections. Applications TestedThe affinity purified 5D3-F7 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human MCP-1 in combination with the biotinylated 2H5 (13-7096) antibody for detection and recombinant human MCP-1 as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/ml - 15 pg/ml should be included in each ELISA plate. The Functional Grade purified 5D3-F7 antibody has been tested for neutralization of MCP-1 bioactivity. References
Peri, G., C. Milanese, C. Matteucci, C., L. Ruco, D. Zhou, S. Sozzani, I. Coletta, and A. Mantovani. 1994. A new monoclonal antibody (5D3-F7) which recognizes human monocyte-chemotactic protein-1 but not related chemokines. Development of a sandwich ELISA and in situ detection of producing cells. J. Immunol. Meth. 174: 249-257. Karrer S, Bosserhoff AK, et al. 2005. The -2518 promotor polymorphism in the MCP-1 gene is associated with systemic sclerosis. J Invest Dermatol. 124(1):92-8. (IHC paraffin, PubMed) Piemonti L, Leone BE, et al. 2002. Human pancreatic islets produce and secrete MCP-1/CCL2: relevance in human islet transplantation. Diabetes. 51(1):55-65. (IHC paraffin, PubMed) Related ProductsCat. 00-0000 eBioscience 10X ELISA Coating Buffer Cat. 44-2404 Nunc MaxiSorp™ flat-bottom 96 well plate Cat. 18-4100 Avidin Horse Radish Peroxidase (AV-HRP) Cat. 00-4202 eBioscience ELISA Diluent Solution, 5X Cat. 00-4203 Super AquaBlue ELISA Substrate Cat. 14-7096 Affinity Purified anti-mouse/human/rat CCL2 (MCP-1, MCP1, MCAF) (clone 2H5) |
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