human CCL2 (MCP-1, MCP1, MCAF) antibody: purified antibody

Contents: Affinity Purified anti-human CCL2 (MCP-1, MCP1, MCAF)
Catalog Number: 14-7099
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: 5D3-F7
Isotype: Mouse IgG1, κ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
14-7099-81	50 ug	$80
14-7099-85	500 ug	$220

*International customers: Please contact your distributor for region specific pricing.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-7099	Affinity Purified anti-human CCL2 (MCP-1, MCP1, MCAF)	N/A	N/A	ELISA cap IC Flow IHC(Paraffin) IP
16-7099	Functional Grade* Purified anti-human CCL2 (MCP-1, MCP1, MCAF)	N/A	N/A	ELISA cap NU
17-7099	Allophycocyanin (APC) anti-human CCL2 (MCP-1, MCP1, MCAF)	633	660	IC Flow
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 5D3-F7 antibody reacts with human, cynomolgus, and rhesus monkey monocyte chemoattractant protein-1 (MCP-1), also known as monocyte chemotactic and activating factor (MCAF) and CCL2. MCP-1 is a CC chemokine which presents as a dimer. Derived from monocytes and vascular endothelial cells, it exerts chemoattractant activity on a variety of cell types, such as monocytes, basophils, T cells and NK cells via chemokine receptors such as CCR2, CCR5, and CCR10.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 5D3-F7 antibody has been reported to be useful for detection of MCP-1 in a sandwich ELISA, neutralization of MCP-1 bioactivity, intracellular staining for flow cytometric analysis, IP, and immunohistochemical staining of frozen tissue sections.

Applications Tested

The affinity purified 5D3-F7 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human MCP-1 in combination with the biotinylated 2H5 (13-7096) antibody for detection and recombinant human MCP-1 as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/ml - 15 pg/ml should be included in each ELISA plate.
The Functional Grade purified 5D3-F7 antibody has been tested for neutralization of MCP-1 bioactivity.

References

Peri, G., C. Milanese, C. Matteucci, C., L. Ruco, D. Zhou, S. Sozzani, I. Coletta, and A. Mantovani. 1994. A new monoclonal antibody (5D3-F7) which recognizes human monocyte-chemotactic protein-1 but not related chemokines. Development of a sandwich ELISA and in situ detection of producing cells. J. Immunol. Meth. 174: 249-257.

Karrer S, Bosserhoff AK, et al. 2005. The -2518 promotor polymorphism in the MCP-1 gene is associated with systemic sclerosis. J Invest Dermatol. 124(1):92-8. (IHC paraffin, PubMed)

Piemonti L, Leone BE, et al. 2002. Human pancreatic islets produce and secrete MCP-1/CCL2: relevance in human islet transplantation. Diabetes. 51(1):55-65. (IHC paraffin, PubMed)

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