Product Information

Contents: Affinity Purified anti-mouse CCL5 (RANTES)
Catalog Number: 14-7085
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: R6G9
Isotype: Mouse IgG1

Immunoblot with anti-mouse CCL5/RANTES (clone R6G9). Raw 264.7 cells were treated with Brefeldin A (lane 1) or LPS and Brefeldin A (lane 2) for 16 hours prior to lysis, SDS-PAGE and immunoblot with R6G9.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-7085	Affinity Purified anti-mouse CCL5 (RANTES)	N/A	N/A	WB

Custom Product Requests
Need a custom product? Download the Custom Product Request Form and submit completed form to customs@ebioscience.com.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

RANTES (Regulated on Activation, Normal T Expressed and Secreted) is an 8kD member of the C-C family of chemkines (CCL5). It is a proinflammatory chemoattractant for several cell types including CD4+ T cells, monocytes and eosinophils. In contrast to other members of the chemokine superfamily, RANTES has been observed to be downregulated upon activation in T cells. In other secreting cells, upregulation is observed in response to activation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This R6G9 antibody has been reported for use in immunoblotting (WB).

Applications Tested

This R6G9 antibody has been tested by immunoblot on lysates of LPS-activated Raw 264.7 cells. This can be used at less than or equal to 2 μg/ml. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Schall TJ. Biology of the RANTES/SIS cytokine family.Cytokine. 1991 May;3(3):165-83.

Danoff TM, Lalley PA, Chang YS, Heeger PS, Neilson EG. Cloning, genomic organization, and chromosomal localization of the Scya5 gene encoding the murine chemokine RANTES. J Immunol. 1994 Feb 1;152(3):1182-9.

Related Products

Cat. 14-4714 Affinity Purified Mouse IgG1, K Isotype Control
Cat. 14-7993 Polyclonal anti-mouse/rat CCL5 (RANTES) (clone Polyclonal)