Product Information

Contents: Affinity Purified anti-mouse interleukin-5 (IL-5)
Catalog Number: 14-7051
Sizes: 50 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: TRFK4
Isotype: Rat IgG2a, κ

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-7051	Affinity Purified anti-mouse IL-5 (Interleukin-5, IL5)	N/A	N/A	ELISA IC Flow
13-7051	Biotin anti-mouse IL-5 (Interleukin-5, IL5)	N/A	N/A	ELISA det ELISPOT

Custom Product Requests
Need a custom product? Download the Custom Product Request Form and submit completed form to customs@ebioscience.com.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The TRFK4 antibody reacts with mouse interleukin-5 (IL-5), also known as BCDF-α and eosinophil differentiation factor (EDF). This 15-20 kDa cytokine is secreted by Th2 cells and exists a homodimer.

Applications Reported

For research use only, not for diagnostic or therapeutic use. TRFK4 has been reported for use in detection of mouse IL-5 by ELISA.

Applications Tested

The purified TRFK4 can be used for blocking of fluorochrome conjugated TRFK4 in intracellular flow cytometry experiments to serve as specificity control. The Biotin conjugated TRFK4 has been tested by sandwich ELISA for detection of mouse IL-5 in combination with TRFK5 and can be used at 1 µg/ml. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Abrams, J. S., M. G. Roncarolo, et al. (1992). "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." Immunol Rev 127: 5-24.
Schumacher, J. H., A. O'Garra, et al. (1988). "The characterization of four monoclonal antibodies specific for mouse IL-5 and development of mouse and human IL-5 enzyme-linked immunosorbent." J Immunol 141(5): 1576-81.

Related Products

Cat. 14-4321 Affinity Purified Rat IgG2a Isotype Control
Cat. 13-4813 Biotin Anti-Rat IgG (clone Polyclonal)