Contents: Affinity Purified anti-mouse interleukin-5 (IL-5)
Catalog Number: 14-7051
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: TRFK4
Isotype: Rat IgG2a, κ
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Description
The TRFK4 antibody reacts with mouse interleukin-5 (IL-5), also known as BCDF-α and eosinophil differentiation factor (EDF). This 15-20 kDa cytokine is secreted by Th2 cells and exists a homodimer.
Applications Reported
For research use only, not for diagnostic or therapeutic use. TRFK4 has been reported for use in detection of mouse IL-5 by ELISA.
Applications Tested
The purified TRFK4 can be used for blocking of fluorochrome conjugated TRFK4 in intracellular flow cytometry experiments to serve as specificity control. The Biotin conjugated TRFK4 has been tested by sandwich ELISA for detection of mouse IL-5 in combination with TRFK5 and can be used at 1 µg/ml. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Abrams, J. S., M. G. Roncarolo, et al. (1992). "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples."
Immunol Rev 127: 5-24.
Schumacher, J. H., A. O'Garra, et al. (1988). "The characterization of four monoclonal antibodies specific for mouse IL-5 and development of mouse and human IL-5 enzyme-linked immunosorbent."
J Immunol 141(5): 1576-81.
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