Product Information

Contents: Affinity Purified anti-human interleukin-4 (IL-4)
Catalog Number: 14-7048
Sizes: 50 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl; 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: MP4-25D2
Isotype: Rat IgG1, κ

Human peripheral blood mononuclear cells were stimulated with PMA and ionomycin in the presence of Brefeldin A for 5 hours. (Left) The cells were surface stained with PE anti-human CD3 (UCHT1) and intracellularly stained with FITC anti-human IL-4 (MP4-25D2). (Right) The cells were surface stained with FITC anti-human CD3 and intracellularly stained with PE anti-human IL-4.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-7048	Affinity Purified anti-human IL-4 (Interleukin-4, IL4)	N/A	N/A	IC Flow
16-7048	Functional Grade* Purified anti-human IL-4 (Interleukin-4, IL4)	N/A	N/A	NU
13-7048	Biotin anti-human IL-4 (Interleukin-4, IL4)	N/A	N/A	ELISA det ELISPOT det
11-7048	FITC anti-human IL-4 (Interleukin-4, IL4)	488	518	IC Flow
12-7048	PE anti-human IL-4 (Interleukin-4, IL4)	488	575	IC Flow
17-7048	APC anti-human IL-4 (Interleukin-4, IL4)	633	660	IC Flow
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

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Description

The MP4-25D2 antibody reacts with human interleukin-4 (IL-4), a 15-19 kDa cytokine secreted by Th2 cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. MP4-25D2 has been reported for use in neutralizing IL-4 binding and biological activity (use Functional Grade purified cat.16-7048). Please refer to the supporting protocols for more information.

Applications Tested

The purified MP4-25D2 can be used for blocking of fluorochrome conjugated MP4-25D2 in intracellular flow cytometry experiments to serve as specificity control. This biotinylated format for this lot of MP4-25D2 has been tested by sandwich ELISA for detection of human IL-4 in combination with 8D4-8 and can be used at 1 μg/ml. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Ramanathan, L., R. Ingram, et al. (1993). Immunochemical mapping of domains in human interleukin 4 recognized by neutralizing monoclonal antibodies. Biochemistry 32(14): 3549-56.
Abrams, J. S., M. G. Roncarolo, et al. (1992). Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev 127: 5-24.

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