Contents: Affinity Purified anti-mouse interleukin-4 (IL-4)
Catalog Number: 14-7042
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: BVD6-24G2
Isotype: Rat IgG1, κ
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Mouse splenocytes were stimulated with ConA for 2 days, followed by IL-2 and IL-4 for 3 days, and restimulated with immobilized anti-CD3 and soluble anti-CD28 in the presence of Brefeldin A for 5 hours. The cells were surface stained with PE anti-mouse CD4 (GK1.5) and intracellularly stained with FITC anti-mouse IL-4 (BVD6-24G2).
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Description
The BVD6-24G2 antibody reacts with mouse interleukin-4 (IL-4), a 14 kDa cytokine secreted by Th2 cells.
Applications Reported
For research use only, not for diagnostic or therapeutic use. BVD6-24G2 is used for detection of mouse IL-4 by ELISA.
Applications Tested
The BVD6-24G2 antibody has been tested by sandwich ELISA for detection of mouse IL-4 in combination with 11B11 and can be used between 0.5-2 µg/ml.
References
Abrams, J. S., M. G. Roncarolo, et al. (1992). "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples."
Immunol Rev 127: 5-24.
Sander, B., I. Hoiden, et al. (1993). "Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining."
J Immunol Methods 166(2): 201-14.
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