Product Information
Contents: Affinity Purified anti-human interleukin-2 (IL-2)
Catalog Number: 14-7029
Sizes: 50 ug, 500 ug
Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C. Avoid repeated freeze/thaw cycles.
Clone: MQ1-17H12
Isotype: Rat IgG2a, κ
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Human peripheral blood mononuclear cells were stimulated with PMA and ionomycin in the presence of Brefeldin A for 5 hours. (Left) The cells were surface stained with PE anti-human CD3 (UCHT1) and intracellularly stained with FITC anti-human IL-2 (MQ1-17H12). (Right) The cells were surface stained with FITC anti-human CD3 and intracellularly stained with PE anti-human IL-2.
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Description
The MQ1-17H12 antibody reacts with human interleukin-2 (IL-2), a 17 kDa T cell growth factor and a major immunoregulatory cytokine. The MQ1-17H12 antibody is a non-neutralizing antibody.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The MQ1-17H12 antibody has been reported for use as capture antibody for ELISA and for intracellular staining for flow cytometric analysis. Fluorochrome conjugated MQ1-17H12 antibody is recommended for use in intracellular staining for flow cytometry.
Applications Tested
The MQ1-17H12 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human Interleukin-2 (IL-2) in combination with the biotin anti-human IL-2 poly (
13-7028) antibody for detection and recombinant human IL-2 (
14-8029) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/ml - 8 pg/ml should be included in each ELISA plate.
References
Andersson, J., J. Abrams, et al. 1994. "Concomitant in vivo production of 19 different cytokines in human tonsils." Immunology 83: 16-24.
Abrams, J. S., M. G. Roncarolo, et al. 1992. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev 127: 5-24.
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