Product Information

Contents: Affinity Purified anti-human interleukin-1β (IL-1β)
Catalog Number: 14-7018
Sizes: 50 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: CRM56
Isotype: Mouse IgG1

Human peripheral blood mononuclear cells were stimulated with LPS in the presence of Brefeldin A for 4 hours. (Left) The cells were surface stained with PE anti-human CD14 (61D3) and intracellularly stained with FITC anti-human IL-1b (CRM56). (Right) The cells were surface stained with FITC anti-human CD14 and intracellularly stained with PE anti-human IL-1b.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-7018	Fluorescein (FITC) anti-human IL-1b (Interleukin-1 beta, IL1b)	488	518	IC Flow
12-7018	Phycoerythrin (PE) anti-human IL-1b (Interleukin-1 beta, IL1b)	488	575	IC Flow
14-7018	Affinity Purified anti-human IL-1b (Interleukin-1 beta, IL1b)	N/A	N/A	ELISA cap IC Flow NU
16-7018	Functional Grade* Purified anti-human IL-1b (Interleukin-1 beta, IL1b)	N/A	N/A	NU
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The CRM56 antibody reacts with human and baboon interleukin-1β (IL-1β). IL-1β is a 17 kDa factor secreted primarily by monocytes. IL-1 has effects on T-helper cells, inducing IL-2 secretion and expression of IL-2 receptors. IL-1 has effects on B cells, promoting cell proliferation and immunoglobulin synthesis.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The CRM56 antibody has been reported for use in intracellular staining for flow cytometric analysis, cytokine neutralization, and ELISA. Fluorochrome conjugated CRM56 is recommended for use in intracellular staining for flow cytometry. Functional Grade purified CRM56, cat. 16-7018, is recommended for use in functional assays.

Applications Tested

The CRM56 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human interleukin-1β (IL-1β) in combination with the biotin CRM57 (13-7016) antibody for detection and recombinant human IL-1β (14-8018) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1 - 4 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/ml - 8 pg/ml should be included in each ELISA plate.

The Functional Grade Purified CRM56 antibody has been tested for neutralization of IL-1β bioactivity.

The fluorochrome-conjugated CRM56 antibody has been tested by intracellular staining for flow cytometric analysis. The fluorochrome- conjugated CRM56 antibody is offered in 2 formats:
- µg size: has been tested by intracellular staining with flow cytometric analysis and can be used at less than or equal to 0.50 µg/million cells/100 ul staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
- test size: has been pre-titrated and tested by intracellular staining and flow cytometric analysis of activated human PBMC and can be used at 20 μl per million cells 100 μl blood.

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