Product Information

Contents: Affinity Purified anti-human interleukin-1 receptor antagonist (IL-1RA)
Catalog Number: 14-7015
Sizes: 50 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: CRM17
Isotype: Mouse IgG1

Human peripheral blood mononuclear cells were stimulated with LPS in the presence of Brefeldin A for 24 hours. (Left) The cells were surface stained with PE anti-human CD14 (61D3) and intracellulary stained with FITC mouse IgG1 isotype control. (Right) The cells were surface stained with PE anti-human CD14 and intracellularly stained with FITC anti-human IL-1RA (CRM17).

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-7015	Affinity Purified anti-human IL-1RA (Interleukin-1 Receptor Antagonist)	N/A	N/A	ELISA cap IC Flow
11-7015	Fluorescein (FITC) anti-human IL-1RA (Interleukin-1 Receptor Antagonist)	488	518	IC Flow

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The CRM17 antibody reacts with human interleukin-1 receptor antagonist (IL-1RA). IL-1RA is produced by macrophages, monocytes, neutrophils, synovial and dermal fibroblasts. The 17 kDa soluble form of IL-1RA (sIL-1RA) is produced by hepatocytes. IL-1RA inhibits the release of both forms of IL-1, the secretion of IL-2, and the expression of IL-2 receptors on the cell surface. IL-1RA also blocks the stimulation of prostaglandin E2 synthesis in synovial cells and the proliferation of thymocytes.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The CRM17 antibody has been reported for use in intracellular staining for flow cytometric analysis, and ELISA capture.

Applications Tested

The CRM17 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human IL-1 receptor antagonist, in combination with the biotinylated rabbit anti-human IL-1RA antibody (13-7014) as the detection antibody and recombinant human IL-1RA as the standard. A suitable range of concentrations of this antibody for ELISA capture is 8-12 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 10,000 pg/ml - 80 pg/ml should be included in each ELISA plate.
The fluorochrome conjugated CRM17 antibody has been tested by intracellular staining and flow cytometric analysis.

Related Products

Cat. 00-0000    eBioscience 10X ELISA Coating Buffer
Cat. 44-2404    Nunc MaxiSorp™ flat-bottom 96 well plate
Cat. 18-4100    Avidin Horse Radish Peroxidase (AV-HRP)
Cat. 00-4202    eBioscience ELISA Diluent Solution, 5X
Cat. 14-4714    Affinity Purified Mouse IgG1, K Isotype Control
Cat. 13-7014    Biotin Rabbit anti-human IL-1RA (Interleukin-1 Receptor Antagonist) (clone Polyclonal)
Cat. 14-8015    Recombinant Human IL-1RA (Interleukin-1 Receptor Antagonist)
Cat. 00-8222    eBioscience IC Fixation Buffer
Cat. 00-8333    eBioscience Permeabilization Buffer(10X)
Cat. 88-8823    eBioscience Fixation & Permeabilization