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Contents: Affinity Purified anti-rat Bax Catalog Number: 14-6999 Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3 Storage Conditions: Store at 4°C. Avoid repeated freeze/thaw cycles. Clone: 1D1 Isotype: Mouse IgG1, κ Prices for This Product* Cat. No. Size Price Qty Action 14-6999-81 50 ug $125 14-6999-82 100 ug $175 *International customers: Please contact your distributor for region specific pricing.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-6999	Affinity Purified anti-rat Bax	N/A	N/A	IP  WB

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

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Description

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The 1D1 monoclonal antibody reacts with rat Bax (~21 kDa), and does not react with human and mouse Bax. 1D1 shows no cross-reaction with Bcl-2 or Bcl-x_L proteins. Bcl-2, Bax, and Bcl-x_L are members of a protein family that regulates apoptosis; while several family members including Bcl-2 and Bcl-x_L inhibit apoptosis, overexpression of Bax promotes programmed cell death. A rat Bax protein peptide sequence, aa 3-16, was used as immunogen.

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Applications Reported

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For research use only, not for diagnostic or therapeutic use. The 1D1 antibody has been reported for use in immunoprecipitation, and immunoblotting (WB).

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Applications Tested

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The 1D1 antibody has been tested by Western Blot and can be used at approximately 1 μg/ml. 1D1 can also be used in IP at approximately 1-10 μg/ml. It is recommended that the reagent be carefully titrated for optimal performance in the assay of interest.

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References

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Hsu YT, et al. PNAS USA (1997) 94(8): 3668-72.
Hsu YT, et. al. Journal of Biological Chemistry (1997) 272(21): 13829-34.

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Related Products

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Cat. 14-6996    Affinity Purified anti-human Bax (clone 2D2)
Cat. 14-6997    Affinity Purified anti-mouse/rat/monkey/human Bax (clone 6A7)
Cat. 14-6998    Affinity Purified anti-mouse Bax (clone 5B7)
Cat. 18-8877    See Catalog # 18-8817 for an alternative product: Mouse TrueBlot™: Horseradish Peroxidase (HRP) anti-mouse IgG