Product Information

Contents: Affinity Purified anti-mouse/human/rat/monkey Bax
Catalog Number: 14-6997
Sizes: 50 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: 6A7
Isotype: Mouse IgG1, κ

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-6997	Affinity Purified anti-mouse/rat/monkey/human Bax	N/A	N/A	WB

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Description

The 6A7 antibody reacts with mouse, rat, monkey and human Bax (21 kDa). The 6A7 antibody shows no cross-reaction with Bcl-2 or Bcl-x_L proteins. Bcl-2, Bax, and Bcl-x_L are members of a protein family that regulates apoptosis; while several family members including Bcl-2 and Bcl-x_L inhibit apoptosis, overexpression of Bax promotes programmed cell death. A human Bax protein peptide sequence, aa 12-24, was used as immunogen.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 6A7 antibody has been reported for use in immunoblotting (WB).

Applications Tested

The 6A7 antibody has been tested by Western Blot and can be used at approximately 1 μg/ml. It is recommended that the reagent be carefully titrated for optimal performance in the assay of interest.

References

Hsu YT, et al. PNAS USA (1997) 94(8): 3668-72.
Hsu YT, et. al. Journal of Biological Chemistry (1997) 272(21): 13829-34.

Related Products

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Cat. 14-6999    Affinity Purified anti-rat Bax (clone 1D1)
Cat. 18-8877    See Catalog # 18-8817 for an alternative product: Mouse TrueBlot™: Horseradish Peroxidase (HRP) anti-mouse IgG