Product Information

Contents: Affinity Purified anti-mouse RORγ(t) (ROR gamma, Retinoid-Related Orphan Receptor gamma)
Catalog Number: 14-6981
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: B2D
Isotype: Rat IgG1, k

Left Image: Mouse thymus (left) and spleen (right) nuclear cell lysates were loaded at 1x10⁵ cells/lane, probed with 2 µg/mL purified B2D and revealed with HRP anti-rat IgG.

Right Image: Untransfected (left) and mouse ROR gamma-transfected (right) 293T nuclear cell lysates were loaded at 1X10⁵ cells/lane, probed with 2 ug/ml purified B2D and revealed with HRP anti-rat IgG.

Note: ROR gamma runs at a slightly higher molecular weight in the transfected cell lysate, compared to the thymus lysate, due to the addition of a tag to full length ROR gamma in the transfected cells.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-6981	Affinity Purified anti-mouse RORg(t) (ROR gamma, Retinoid-Related Orphan Receptor gamma)	N/A	N/A	WB

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Description

The B2D monoclonal antibody reacts with the mouse RORγ(t) protein. RORγ is a member of the retinoic acid-related orphan receptor (ROR) family, which also includes RORα and RORβ. ROR family proteins are ligand-dependent transcription factors that play roles in multiple physiological processes. RORγ is expressed in several tissues including liver, lung, muscle, heart and kidney. Furthermore, it was discovered that alternative transcription results in the expression of an isoform, RORγt, which is expressed exclusively in cells of the lymphoid compartment, namely CD4+CD8+ “double-positive” thymoyctes, Th17 cells of the periphery and lymphoid tissue inducer (Lti) cells of lymphoid organs.

The RORγt isoform differs from RORγ by three unique amino acids at the amino terminus. Therefore, the B2D antibody will react with both the RORγ and RORγt isoforms.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This B2D antibody has been reported for use in immunoblotting (WB).

Applications Tested

This B2D antibody has been tested by western blot analysis of mouse thymus nuclear lysates. This B2D antibody can be used at a starting concentration of 2 ug/ml, however the optimal concentration should be determined for individual experimental conditions.

References

Medvedev A, Yan ZH, Hirose T, Giguère V, Jetten AM. Cloning of a cDNA encoding the murine orphan receptor RZR/ROR gamma and characterization of its response element. Gene. 1996 Nov 28; 181(1-2):199-206.

He YW, Deftos ML, Ojala EW, Bevan MJ. RORgamma t, a novel isoform of an orphan receptor, negatively regulates Fas ligand expression and IL-2 production in T cells. Immunity. 1998 Dec;9(6):797-806.

Sun Z, Unutmaz D, Zou YR, Sunshine MJ, Pierani A, Brenner-Morton S, Mebius RE, Littman DR. Requirement for RORgamma in thymocyte survival and lymphoid organ development. Science. 2000 Jun 30;288(5475):2369-73.

Ivanov II, McKenzie BS, Zhou L, Tadokoro CE, Lepelley A, Lafaille JJ, Cua DJ, Littman DR. The orphan nuclear receptor RORgammat directs the differentiation program of proinflammatory IL-17+ T helper cells. Cell. 2006 Sep 22;126(6):1121-33.

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