Also known as: RORgamma, RORg, RORg(t), Retinoid-Related Orphan Receptor gamma
RUO: For Research Use Only
Left: Mouse thymus (left) and spleen (right) nuclear cell lysates were loaded at 1x105 cells/lane, probed with 2 µg/mL of Anti-Mouse RORγ (t) Purified and revealed with Anti-Rat IgG HRP. Right: Untransfected (left) and mouse RORγ-transfected (right) 293T nuclear cell lysates were loaded at 1X105 cells/lane, probed with 2 µg/ml of Anti-Mouse RORγ (t) Purified and revealed with HRP anti-rat IgG.
Note: RORγ runs at a slightly higher molecular weight in the transfected cell lysate, compared to the thymus lysate, due to the addition of a tag to full length RORγ in the transfected cells.
Contents: Anti-Mouse ROR gamma (t) Purified Catalog Number: 14-6981 Concentration: 0.5 mg/ml Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3 Storage Conditions: Store at 2-8°C. Clone: B2D Host/Isotype: Rat IgG1, k
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Size
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14-6981-80
25 ug
14-6981-82
100 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The B2D monoclonal antibody reacts with the mouse RORγ(t) protein. RORγ is a member of the retinoic acid-related orphan receptor (ROR) family, which also includes RORα and RORβ. ROR family proteins are ligand-dependent transcription factors that play roles in multiple physiological processes. RORγ is expressed in several tissues including liver, lung, muscle, heart and kidney. Furthermore, it was discovered that alternative transcription results in the expression of an isoform, RORγt, which is expressed exclusively in cells of the lymphoid compartment, namely CD4+CD8+ “double-positive” thymoyctes, Th17 cells of the periphery and lymphoid tissue inducer (Lti) cells of lymphoid organs.
The RORγt isoform differs from RORγ by three unique amino acids at the amino terminus. Therefore, the B2D antibody will react with both the RORγ and RORγt isoforms.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This B2D antibody has been reported for use in immunoblotting (WB).
Applications Tested
This B2D antibody has been tested by western blot analysis of mouse thymus nuclear lysates. This B2D antibody can be used at a starting concentration of 2 ug/ml, however the optimal concentration should be determined for individual experimental conditions.
References
Sanos SL, Bui VL, Mortha A, Oberle K, Heners C, Johner C, Diefenbach A. RORgammat and commensal microflora are required for the differentiation of mucosal interleukin 22-producing NKp46+ cells. Nat Immunol. 2009 Jan;10(1):83-91. (B2D, IF, IHC frozen, PubMed)
Medvedev A, Yan ZH, Hirose T, Gigučre V, Jetten AM. Cloning of a cDNA encoding the murine orphan receptor RZR/ROR gamma and characterization of its response element. Gene. 1996 Nov 28; 181(1-2):199-206.
He YW, Deftos ML, Ojala EW, Bevan MJ. RORgamma t, a novel isoform of an orphan receptor, negatively regulates Fas ligand expression and IL-2 production in T cells.
Immunity. 1998 Dec;9(6):797-806.
Sun Z, Unutmaz D, Zou YR, Sunshine MJ, Pierani A, Brenner-Morton S, Mebius RE, Littman DR. Requirement for RORgamma in thymocyte survival and lymphoid organ development. Science. 2000 Jun 30;288(5475):2369-73.
Ivanov II, McKenzie BS, Zhou L, Tadokoro CE, Lepelley A, Lafaille JJ, Cua DJ, Littman DR. The orphan nuclear receptor RORgammat directs the differentiation program of proinflammatory IL-17+ T helper cells.
Cell. 2006 Sep 22;126(6):1121-33.
