Product Information

Contents: Affinity Purified anti-human RASSF1A
Catalog Number: 14-6888
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eB114-10H1
Isotype: Mouse IgG1, κ

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-6888	Affinity Purified anti-human RASSF1A	N/A	N/A	IP WB

Custom Product Requests
Need a custom product? Download the Custom Product Request Form and submit completed form to customs@ebioscience.com.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eB114 antibody reacts with the RASSF1A isoform of the tumor suppressor gene RASSF1. RASSF1A contains a Ras Association Domain (RA) and binds Ras in a GTP-dependent manner to effect apoptosis. The RASSF1A isoform undergoes epigenetic inactivation in a variety of human primary tumors. This has been associated with hypermethylation of a CpG island(s) in its promoter region. This antibody was generated against a synthetic peptide corresponding to the splice junction region of RASSF1A.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The eB114 antibody has been reported for use in immunoprecipitation, and immunoblotting (WB).

Applications Tested

The eB114-10H1 has been tested by Western blotting and can be used at approximately 1-10 μg/ml. RASS1A is detected as a 45 kDa band in Western blotting of a HeLa cell extract. It is recommended that the reagent be carefully titrated for optimal performance in the assay of interest.

References

Dallol, A., Agathanggelou, A., Fenton, S.L., Ahmed-Choudhury, J., Hesson, L., Vos, M.D., Geoffrey J. Clark, G. J., Downward, J., Eamonn R. Maher, E.R., and F. Latif. 2004. RASSF1A Interacts with Microtubule-Associated Proteins and Modulates Microtubule Dynamics. Cancer Research 64, 4112-4116. (eb114-10H1, WB, PubMed)

Whang, Y.M., Kim, Y.H., Kim, J.S. and Y.D. Yoo. 2005. RASSF1A Suppresses the c-Jun-NH2-Kinase Pathway and Inhibits Cell Cycle Progression. Cancer Research 65, 3682-3690. (eb114-10H1, WB, PubMed)

Dammann R, Yang G, Pfeifer GP. (2001) "Hypermethylation of the cpG island of Ras association domain family 1A (RASSF1A), a putative tumor suppressor gene from the 3p21.3 locus, occurs in a large percentage of human breast cancers." Cancer Res. 61(7):3105-9.

Ortiz-Vega S, Khokhlatchev A, Nedwidek M, Zhang XF, Dammann R, Pfeifer GP, Avruch J. (2002). "The putative tumor suppressor RASSF1A homodimerizes and heterodimerizes with the Ras-GTP binding protein Nore1." Oncogene. (9):1381-90.

Related Products

Cat. 14-4714 Affinity Purified Mouse IgG1, K Isotype Control
Cat. 18-8877 See Catalog # 18-8817 for an alternative product: Mouse TrueBlot™: Horseradish Peroxidase (HRP) anti-mouse IgG