Contents: Anti-Human/Mouse WASP Purified Catalog Number: 14-6887 Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3 Storage Conditions: Store at 2-8°C. Host/Isotype: Goat IgG
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14-6887-63
10 ug
14-6887-81
50 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa FluorŪ 647 conjugated products with eFluorŪ 660 conjugated products.
Description
This polyclonal antibody reacts with Wiskott-Aldrich syndrome protein (WASP), which is a cytosolic adaptor protein mediating the rearrangement of the actin cytoskeleton upon surface receptor signaling. WASP is a 54 kDa protein belonging to the WASP/SCAR/WAVE family of proteins, and is expressed selectively in hematopoietic lineages. Mutations in WASP are associated with diseases of the hematopoietic/immunologic system.
Applications Reported
For research use only, not for diagnostic or therapeutic use. Purified Human/Mouse WASP Polyclonal has been reported for use in immunoblotting (WB), immunohistochemical staining, and ELISA.
Applications Tested
Purified Human/Mouse WASP Polyclonal has been tested by western blot analysis of K562 cell lysates. As a starting dilution, it is recommended that this polyclonal antibody be used at 2 μg/ml.
References
Derry JM, Ochs HD, Francke U.
Isolation of a novel gene mutated in Wiskott-Aldrich syndrome.
Cell. 1994 Aug 26;78(4):635-44.
Derry JM, Wiedemann P, Blair P, Wang Y, Kerns JA, Lemahieu V, Godfrey VL, Wilkinson JE, Francke U.
The mouse homolog of the Wiskott-Aldrich syndrome protein (WASP) gene is highly conserved and maps near the scurfy (sf) mutation on the X chromosome.
Genomics. 1995 Sep 20;29(2):471-7.
Symons M, Derry JM, Karlak B, Jiang S, Lemahieu V, Mccormick F, Francke U, Abo A.
Wiskott-Aldrich syndrome protein, a novel effector for the GTPase CDC42Hs, is implicated in actin polymerization.
Cell. 1996 Mar 8;84(5):723-34.
