Product Information

Contents: Affinity Purified anti-mouse/human/rat PKC
Catalog Number: 14-6793
Formulation: 200 µg/ml of mouse monoclonal IgG2a in PBS containing 0.1 % sodium azide and 0.2% gelatin.
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: MC5
Isotype: Mouse IgG2a, κ

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications

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Description

The MC5 antibody reacts with mouse, rat, and human PKC; the antibody was derived from the fusion of X-63 myeloma cells with spleen cells from a BALB/c mouse immunized with protein kinase C purified from bovine brain. To date at least 12 different protein kinase C (PKC) isoforms have been identified that possess distinct differences in structure, substrate requirement, expression and localization. The PKC family of serine/threonine protein kinases is involved in complex intracellular signaling processes that are mediated though phospholipid hydrolysis. The general structure of a PKC molecule consists of a catalytic and a regulatory domain; composed of a number of conserved regions interspersed with variable domains. The PKC isoforms have been divided into three groups, conventional PKC isoforms (α, βI, βII and γ), novel PKC isoforms (δ, ε, η, θ and μ), and atypical PKC isoforms (ζ, and λ/ι). The more recently identified PKCν shows the most sequence homology to PKCμ. The conventional PKC isoforms require calcium and diacylglycerol (DAG) for activation, the novel isoforms require DAG, and the atypical PKC isoforms require neither calcium nor DAG.
The antibody MC5 reacts with PKC alpha, beta, and gamma of mouse, rat and human origin.

Applications Reported

For research use only, not for diagnostic or therapeutic use. Purified anti-mo/hu/rat PKC has been reported for use in immunoprecipitation, and immunoblotting (WB).

Applications Tested

The MC5 antibody has been tested by immunoblotting (WB). (1:200 starting dilution). It is recommended that this antibody be titrated for optimal performance in the assay of interest.

References

Van der Zee EA, Bolhuis JJ, Solomonia RO, Horn G, Luiten PG. 1995. Differential distribution of protein kinase C (PKC alpha beta and PKC gamma) isoenzyme immunoreactivity in the chick brain. Brain Res. 676(1):41-52.
Ferro TJ, Parker DM, Commins LM, Phillips PG, Johnson A. 1993. Tumor necrosis factor-alpha activates pulmonary artery endothelial protein kinase C. Am J Physiol. 264(1 Pt 1): L7-14.
Young S, Rothbard J, Parker PJ. 1988. A monoclonal antibody recognising the site of limited proteolysis of protein kinase C. Inhibition of down-regulation in vivo. Eur J Biochem. 173(1):247-52.

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