Product Information

Contents: Affinity Purified anti-mouse, human, hamster cyclin B1
Catalog Number: 14-6792
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: V152
Isotype: Mouse IgG1

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications

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Description

The V152 antibody reacts with Cyclin B1; the antibody was derived from the fusion of Sp2 myeloma cells with spleen cells from a mouse immunized with a polyhistidine tagged hamster cyclin B1. During each cell cycle cyclins undergo periodic accumulation and destruction. As key regulators of the cell cycle the cyclins control important transitions by activating Cdks (1,2). Cyclin A regulates at least two cdks, Cdc2 p34 and Cdk2, and is believed to be necessary for progression through S phase and is active during the G2-M transition (1&3,4). Cyclin B1 also associates with Cdc2 p34 and this complex is requisite for the G2-M progression. The Cyclin B1/Cdc2 p34 complex is known as MPF, M-phase kinase, maturation-promoting factor, or M-phase specific histone kinase, is widely accepted as the trigger of mitosis in all organisms (5-9).

Applications Reported

For research use only, not for diagnostic or therapeutic use. Purified anti-mouse, human, hamster cyclin B1 has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistochemical staining.

Applications Tested

The V152 antibody has been tested by immunoblotting (WB). (1:200 starting dilution). It is recommended that this antibody be titrated for optimal performance in the assay of interest.

References

1. Bandara LR, Adamczewski JP, Zamanian M, Poon RY, Hunt T, Thangue NB. 1992. Cyclin A recruits p33cdk2 to the cellular transcription factor DRTF1. J Cell Sci Suppl 16: 77-85.
2. Desdouets C, Sobczak-Thepot J, Murphy M, Brechot C. 1995. Cyclin A: Function and expression during cell proliferation. Prog Cell Cycle Res 1:115-123.
3. Brechot C. 1993. Oncogenic activation of cyclin A. Curr Opin Genet Dev 3(1): 11-18.
4. Goldstone S, Pavey S, Forrest A, Sinnamon J, Gabrielli B. 2001. Cdc25-dependent activation of cyclin A/cdk2 is blocked in G2 phase arrested cells independently of ATM/ATR. Oncogene 20(8): 921-932.
5. Liu D, Liao C, Wolgemuth DJ. 2000. A role for cyclin A1 in the activation of MPF and G2-M transition during meiosis of male germ cells in mice. Dev Biol 224(2): 338-400.
6. Hoffman I, Clarke PR, Marcotte MJ, Karsenti E, Draetta G. 1993. Phosphorylation and activation of human cdc25-C by cdc2-cyclin B and its involvement in the self-amplification of MPF at mitosis. EMBO J 12(1): 53-63.
7. Kishimoto T, Okumura E. 1997. In vivo regulation of the entry into M-phase: initial activation and nuclear translocation of cyclin B/Cdc2. Prog Cell Cycle Res 3:241-249.
8. Baratte B, Meijer L, Galaktionov K, Beach D. 1992. Screening for antimitotic compounds using the cdc25 tyrosine phosphatase, an activator of the mitosis-inducing p34cdc2/cyclin Bcdc13 protein kinase. Anticancer Res 12(3): 873-880.
9. Thron CD. 1994. Theoretical dynamics of the cyclin B-MPF system: a possible role for p13suc1. Biosystems 32(2):97-109.

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