Product Information

Contents: Affinity Purified anti-murine, human, bovine cyclin A
Catalog Number: 14-6790
Formulation: 200 µg/ml mouse monoclonal IgG2a in PBS containing 0.1 % sodium azide and 0.2% gelatin.
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: E23.1
Isotype: Mouse IgG2a, κ

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications

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Description

The E67.1 antibody reacts with mouse, human, and bovine cyclin A; the antibody was derived from a mouse immunized with bovine cyclin A. The epitope for this antibody has been mapped to a region containing the sequence LDYP. This epitope maps within amino acids 144-148 of human cyclin A2.Cyclin As that contain this epitope: Cow cyclin A2, human cyclin A2, hamster cyclin A2, mouse cylin A2.Cyclin As that do not contain this epitope: Chicken cyclin A2, human cyclin A1, mouse cyclin A1. During each cell cycle cyclins undergo periodic accumulation and destruction. As key regulators of the cell cycle the cyclins control important transitions by activating Cdks (1,2). Cyclin A regulates at least two cdks, Cdc2 p34 and Cdk2, and is believed to be necessary for progression through S phase and is active during the G2-M transition (1&3,4). Cyclin B1 also associates with Cdc2 p34 and this complex is requisite for the G2-M progression. The Cyclin B1/Cdc2 p34 complex is known as MPF, M-phase kinase, maturation-promoting factor, or M-phase specific histone kinase, is widely accepted as the trigger of mitosis in all organisms (5-9).

Applications Reported

For research use only, not for diagnostic or therapeutic use. Purified anti-murine, human, bovine cyclin A has been reported for use in immunoprecipitation, immunoblotting (WB), and immunohistochemical staining.

Applications Tested

The E67.1 antibody has been tested by immunoblotting (WB). (1:100 starting dilution). It is recommended that this antibody be titrated for optimal performance in the assay of interest.

References

1. Bandara LR, Adamczewski JP, Zamanian M, Poon RY, Hunt T, Thangue NB. 1992. Cyclin A recruits p33cdk2 to the cellular transcription factor DRTF1. J Cell Sci Suppl 16: 77-85.
2. Desdouets C, Sobczak-Thepot J, Murphy M, Brechot C. 1995. Cyclin A: Function and expression during cell proliferation. Prog Cell Cycle Res 1:115-123.
3. Brechot C. 1993. Oncogenic activation of cyclin A. Curr Opin Genet Dev 3(1): 11-18.
4. Goldstone S, Pavey S, Forrest A, Sinnamon J, Gabrielli B. 2001. Cdc25-dependent activation of cyclin A/cdk2 is blocked in G2 phase arrested cells independently of ATM/ATR. Oncogene 20(8): 921-932.
5. Liu D, Liao C, Wolgemuth DJ. 2000. A role for cyclin A1 in the activation of MPF and G2-M transition during meiosis of male germ cells in mice. Dev Biol 224(2): 338-400.
6. Hoffman I, Clarke PR, Marcotte MJ, Karsenti E, Draetta G. 1993. Phosphorylation and activation of human cdc25-C by cdc2-cyclin B and its involvement in the self-amplification of MPF at mitosis. EMBO J 12(1): 53-63.
7. Kishimoto T, Okumura E. 1997. In vivo regulation of the entry into M-phase: initial activation and nuclear translocation of cyclin B/Cdc2. Prog Cell Cycle Res 3:241-249.
8. Baratte B, Meijer L, Galaktionov K, Beach D. 1992. Screening for antimitotic compounds using the cdc25 tyrosine phosphatase, an activator of the mitosis-inducing p34cdc2/cyclin Bcdc13 protein kinase. Anticancer Res 12(3): 873-880.
9. Thron CD. 1994. Theoretical dynamics of the cyclin B-MPF system: a possible role for p13suc1. Biosystems 32(2):97-109.

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