human c-Myc antibody, Affinity Purified conjugate

Contents: Affinity Purified anti-human c-Myc p67
Catalog Number: 14-6784
Formulation: 200 µg/ml mouse monoclonal IgG1 in PBS containing 0.1 % sodium azide and 0.2% gelatin.
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: 9E10
Isotype: Mouse IgG1

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14-6784-80

25 ug

14-6784-82

100 ug

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-6784	Purified anti-human c-Myc p67	N/A	N/A	IHC IP WB

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Description

The 9E10 antibody reacts with human c-Myc p67; the antibody was raised against a synthetic peptide corresponding to amino acids 408-439 within the carboxy terminal domain of human c-Myc. This antibody can be used to detect myc-tagged protein.
The transcription factor c-Myc is a proto-oncogene that is at the focal point in cell cycle regulation, metabolism, apoptosis, differentiation, cell adhesion, and tumorigenesis (1-3). In normal cells the expression of c-Myc is tightly regulated but in human cancers c-Myc is frequently deregulated (2&3).c-Myc also plays a pivotal role in apoptosis, most notably its connections to the CD95/Fas death receptor pathway (1&4). These different biological responses to c-Myc are most likely the result of different overlapping subsets of c-Myc target genes (1).

Applications Reported

For research use only, not for diagnostic or therapeutic use. Purified anti-human c-Myc p67 has been reported for use in immunoprecipitation, immunoblotting (WB), and immunohistochemical staining.

Applications Tested

The 9E10 antibody has been tested by immunoblotting (WB).

References

1. Hoffman B, Amanullah A, Shafarenko M, Liebermann DA. 2002. The proto-oncogene c-myc in hematopoietic development and leukemogenesis. Oncogene 21(21): 3414-3421.
2. Boxer LM, Dang CV. 2001. Translocations involving c-myc and c-myc function. Oncogene 20(40):5595-5610.
3. Dang CV, Resar LM, Emison E, Kim S, Li Q, Prescott JE, Wonsey D, Zeller K. 1999. Function of the c-Myc oncogenic transcription factor. Exp Cell Res 253(1): 63-77.
4. Prendergast GC. 1999. Mechanisms of apoptosis by c-Myc. Oncogene 18(19):2967-2987.
5. Hillman MC, Yang LS, Sun S, Duke JL, O'Neil KT, Kochie JE, Karjoo A, Nath P, Breth LA, Murphy K, Ross OH, Burn TC, Hollis GF, Wynn R. 2001. A comprehensive system for protein purification and biochemical analysis based on antibodies to c-myc peptide. Protein Expr Purif. 23(2):359-68.
6. Xie LY, Abou-Samra AB. 1998. Epitope tag mapping of the extracellular and cytoplasmic domains of the rat parathyroid hormone (PTH)/PTH-related peptide receptor. Endocrinology. 139(11):4563-7.
7. Robertson D, Paterson HF, Adamson P, Hall A, Monaghan P. 1995. Ultrastructural localization of ras-related proteins using epitope-tagged plasmids. J Histochem Cytochem. 43(5):471-80.
8. Hilpert K, Hansen G, Wessner H, Kuttner G, Welfle K, Seifert M, Hohne W. 2001. Anti-c-myc antibody 9E10: epitope key positions and variability characterized using peptide spot synthesis on cellulose. Protein Eng. 14(10):803-6.

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