Product Information

Contents: Affinity Purified anti-mouse/human/rat origin Rb
Catalog Number: 14-6783
Formulation: 200 µg/ml mouse monoclonal IgG1 in PBS containing 0.1 % sodium azide and 0.2% gelatin.
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: 1F8
Isotype: Mouse IgG1, κ

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications

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Description

The 1F8 antibody reacts with mouse, rat, and human Rb; the antibody was derived by fusion of spleen cells from a mouse immunized with a human recombinant full length Rb protein with Sp2/0-Ag14 myeloma cells. The pocket protein family consists of three structurally and functionally related proteins, Rb, p107, and p130 (1). This family of tumor suppressors function to regulate important cellular transcription factors, such as the E2F family (1,2). The E2F proteins regulate the expression of genes whose products are important for cell cycle progression. The inactivation Rb is catalyzed by CDK phosphorylation thereby releasing E2F during the G1-S phase cellular progression (3). Unchecked inactivation of Rb in G1 phase has been indicated as a universal mechanism underlying cellular transformation (4,5). While Rb has been the most studied among the pocket proteins, p107 and p130 have also been shown to be key regulators of E2F (6). Several studies have also provided evidence that p107/p130 provide different functions in E2F regulation than does Rb (6,7). Rb, p107, and p130 each contain a conserved ?A/B pocket?, which is the target of several viral oncoproteins, namely SV40 large T-antigen and adenovirus E1A (8).

Applications Reported

For research use only, not for diagnostic or therapeutic use. Purified anti-mo/hu/rat origin Rb has been reported for use in immunoprecipitation, immunoblotting (WB), and immunohistochemical staining.

Applications Tested

The 1F8 antibody has been tested by immunoblotting (WB). (1:100 starting dilution). It is recommended that this antibody be titrated for optimal performance in the assay of interest.

References

1. Halaban R. 1999. Melanoma cell autonomous growth: the Rb/E2F pathway. Cancer Metastasis Rev 18(3):333-343.
2. Sidle A., Palaty C., Dirks P., Wiggan O., Kiess M., Gill R.M., Wong A.K., Hamel P.A. 1996. Activity of the retinoblastoma family proteins, pRB, p107, and p130, during cellular proliferation and differentiation. Crit Rev Biochem Mol Biol 31(3):237-271.
3. Classon M., Dyson N. 2001. p107 and p130: versatile proteins with interesting pockets. Exp Cell Res 264(1):135-147.
4. Hatakeyama M., Weinberg R.A. 1995. The role of RB in cell cycle control. Prog Cell Cycle Res 1:9-19.
5. Nevins J.R. 2001. The Rb/E2F pathway and cancer. Hum Mol Genet 10(7):699-703.
6. Hurford R.K., Cobrinik D., Lee M.H., Dyson N. 1997. pRB and p107/p130 are required for the regulated expression of different sets of E2F responsive genes. Genes Dev 11(11):1447-1463.
7. Smith E.J., Leone G., Nevins J.R. 1998. Distinct mechanisms control the accumulation of the Rb-related p107 and p130 proteins during cell growth. Cell Growth Differ 9(4):297-303.
8. Knudsen E.S., Wang J.Y. 1998. Hyperphosphorylated p107 and p130 bind to T-antigen: identification of a critical regulatory sequence present in RB but not in p107/p130. Oncogene 16(13):1655-1663.

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