Contents: Affinity Purified Anti-GFP and GFP mutant fusion proteins polyclonal
Catalog Number: 14-6758
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: Polyclonal
Isotype: Rabbit IgG
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Description
The polyclonal antibody reacts with GFP; it was raised against a peptide mapping near the carboxy terminus of aequorea victoria GFP. Green fluorescent protein was originally cloned from the cnidarian, Aequorea victoria. This exceptional protein absorbs blue light (maximally at 395 nm) and emits green light (peak at 509 nm) without the requirement of exogenous substrates and cofactors (1). These unique qualities allow GFP to be used to monitor gene expression and protein localization in vivo. Several mutant forms of GFP have been developed which fluoresce more intensely and have shifted excitation maxima when compared to the wild type GFP, making them useful for FACS, fluorescence microscopy, and double-labeling applications (2,3).
Applications Reported
For research use only, not for diagnostic or therapeutic use. This Polyclonal antibody has been reported for use in immunoprecipitation, and immunoblotting (WB).
Applications Tested
Purified Anti-GFP and GFP mutant fusion proteins poly has been tested by immunoblotting (WB).
References
1. Chalfie M, Tu Y., Euskirchen G., Ward W.W., Prasher D.C. 1994. Green Fluorescent Protein as a Marker for Gene Expression. Science 263: 802-805.
2. Cormack B.P., Valdivia R.H., and Falkow S. 1996. FACS-optimized mutants of the green fluorescent protein (GFP). Gene 173: 33-38.
3. Rizzuto R., Brini M., De Giorgi F., Rossi R., Heim R., Tsien R.Y., and Pozzan T. 1996. Double labelling of the subcellular structures with organelle-targeted GFP mutants in vivo. Curr.Biol. 6:183-188.
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