Contents: Affinity Purified anti-human c-Myc p67 polyclonal
Catalog Number: 14-6755
Formulation: 200 µg/ml rabbit polyclonal IgG in PBS containing 0.1 % sodium azide and 0.2% gelatin.
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: Polyclonal
Isotype: Rabbit IgG
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Description
The polyclonal antibody reacts with c-Myc p67; the antibody was raised against a synthetic peptide mapping near the carboxy terminal domain of human c-Myc. The transcription factor c-Myc is a proto-oncogene that is at the focal point in cell cycle regulation, metabolism, apoptosis, differentiation, cell adhesion, and tumorigenesis (1-3). In normal cells the expression of c-Myc is tightly regulated but in human cancers c-Myc is frequently deregulated (2&3).c-Myc also plays a pivotal role in apoptosis, most notably its connections to the CD95/Fas death receptor pathway (1&4). These different biological responses to c-Myc are most likely the result of different overlapping subsets of c-Myc target genes (1).
Applications Reported
For research use only, not for diagnostic or therapeutic use. Purified anti-human c-Myc p67 poly has been reported for use in immunoprecipitation, immunoblotting (WB), and immunohistochemical staining.
Applications Tested
Purified anti-human c-Myc p67 poly has been tested by immunoblotting (WB). (1:200 starting dilution). It is recommended that this antibody be titrated for optimal performance in the assay of interest.
References
1. Hoffman B, Amanullah A, Shafarenko M, Liebermann DA. 2002. The proto-oncogene c-myc in hematopoietic development and leukemogenesis. Oncogene 21(21): 3414-3421.
2. Boxer LM, Dang CV. 2001. Translocations involving c-myc and c-myc function. Oncogene 20(40):5595-5610.
3. Dang CV, Resar LM, Emison E, Kim S, Li Q, Prescott JE, Wonsey D, Zeller K. 1999. Function of the c-Myc oncogenic transcription factor. Exp Cell Res 253(1): 63-77.
4. Prendergast GC. 1999. Mechanisms of apoptosis by c-Myc. Oncogene 18(19):2967-2987.
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