Contents: Affinity Purified anti-mouse/human/rat AKT polyclonal
Catalog Number: 14-6753
Formulation: 200 µg/ml rabbit polyclonal IgG in PBS containing 0.1 % sodium azide and 0.2% gelatin.
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: Polyclonal
Isotype: Rabbit IgG
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Description
The polyclonal antibody reacts with mouse, human and rat AKT; the antibody was raised against a synthetic peptide mapping to the carboxy terminal domain of human Akt1. Akt1, also known as Protein kinase B, is a serine/threonine kinase that is at the center of many signal transduction pathways. Akt1 regulates glucose metabolism, transcription, apoptosis, cell proliferation, angiogenesis, and cell motility (1). The activation of Akt1 occurs downstream of PI-3K, which upon activation by an extracellular molecule, generates PIP (3) (2). PIP (3) is a lipid second messenger that promotes the translocation of Akt1 to the plasma membrane via PH (pleckstrin-homology) domain binding (2-4). At the plasma membrane Akt1 is phosphorylated and activated by PDK-1 at its catalytic site on residue Thr308 (1). Residue Ser473 is also phosphorylated at this time but the kinase has not yet been identified (1). In this activated form Akt1 phosphorylates proteins that contain the motif, R-x-R-x-x-S/T-F/L, at the plasma membrane, in the cytosol, and in the nucleus (1). In addition to its kinase activity, Akt1 forms complexes with other proteins that are not substrates. These other proteins act as modulators of Akt1 activity (1).
Applications Reported
For research use only, not for diagnostic or therapeutic use. Purified anti-mo/hu/rat AKT poly has been reported for use in immunoblotting (WB), and immunohistochemical staining.
Applications Tested
Purified anti-mo/hu/rat AKT poly has been tested by immunoblotting (WB). A recommended starting dilution for immunoblotting is 1/200.
References
1. Brazil DP, Park J, Hemmings BA. 2002. PKB binding proteins. Getting in on the Akt. Cell 111 (3):293-303.
2. Nicholson KM, Anderson NG. 2002. The protein kinase B/Akt signalling pathway in human malignancy. Cell Signal 14(5): 381-395.
3. Marte BM, Downward J. 1997. PKB/Akt: connecting phosphoinositide 3-kinase to cell survival and beyond. Trends Biochem Sci 22(9): 355-358.
4. Coffer PJ, Jin J, Woodgett JR. 1998. Protein kinase B (c-Akt): a multifunctional mediator of phosphatidylinositol 3-kinase activation. Biochem J. 335(Pt1): 1-13.
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