Product Information

Contents: Affinity Purified anti-mouse/human/rat PCNA polyclonal
Catalog Number: 14-6748
Sizes: 10 ug, 50 ug
Formulation: 200 µg/ml rabbit polyclonal IgG in PBS containing 0.1 % sodium azide and 0.2% gelatin.
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: Polyclonal
Isotype: Rabbit IgG1

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-6748	Purified anti-mouse/human/rat PCNA polyclonal	N/A	N/A	IHC IP WB

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The polyclonal antibody reacts with mouse, rat, and human PCNA; the antibody was raised against a peptide mapping to the carboxy terminus of human PCNA. The evolutionarily conserved protein PCNA, or cyclin, was first characterized as a DNA polymerase accessory protein. It functions as a DNA sliding clamp for DNA polymerase delta and epsilon, and is an key component of for eukaryotic chromosomal DNA replication (1-3). In more recent research PCNA has been linked to an increasing number of cellular functions, these include cell cycle regulation, DNA repair, Okazaki fragment joining, DNA methylation, and chromatin assembly (2,4, &5). In cell cycle regulation p21 interacts with PCNA, which inhibits DNA replication (2,5). It has also been described that high levels of PCNA in the presence of p53 will lead to DNA repair (4). PCNA is also a very useful marker of cellular proliferation as it is seen most prominently expressed during the S phase of the cell cycle (6).

Applications Reported

For research use only, not for diagnostic or therapeutic use. Purified anti-mo/hu/rat PCNA poly has been reported for use in immunoprecipitation, immunoblotting (WB), and immunohistochemical staining.

Applications Tested

Purified anti-mo/hu/rat PCNA poly has been tested by immunoblotting (WB). (1:200 starting dilution). It is recommended that this antibody be titrated for optimal performance in the assay of interest.

References

1. Kelman Z. 1997. PCNA: structure, functions and interactions. Oncogene 14(6):629-40.
2. Tsurimoto T. 1999. PCNA binding proteins. Front Biosci 4:D849-D858.
3. Balajee A.S., Geard C.R. 2001. Chromatin-bound PCNA complex formation triggered by DNA damage occurs independent of the ATM gene product in human cells. Nucleic Acid Res 29(6):1341-1351.
4. Pauneshku T., Mittal S., Protic M., Oryhon J., Korolev S.V., Joachimiak A., Woloschak G.E. 2001. Proliferating cell nuclear antigen (PCNA): ringmaster of the genome. Int J Radiat Biol 77(10):1007-1021.
5. Rossig L., Jadidi A.S., Urbich C., Badorff C., Zeiher A.M., Dimmeler S. 2001. Akt-dependent phosphorylation of p21(Cip1) regulates PCNA binding and proliferation of endothelial cells. Mol Cell Biol 21(16):5644-5657.
6. Celis J.E., Madsen P., Nielsen S., Celis A. 1986. Nuclear patterns of cyclin (PCNA) antigen distribution subdivide S-phase in cultured cells?some applications of PCNA antibodies. Leuk Res 10(3):237-249.

Related Products

Cat. 66-P748 Blocking Peptides for anti-mouse/human/rat PCNA polyclonal