14-6742

Contents: Affinity Purified anti-human Smac/Diablo polyclonal
Catalog Number: 14-6742
Formulation: Smac/DIABLO (C17) is provided as an affinity purified rabbit polyclonal antibody, raised against a peptide mapping to the carboxy terminus of human Smac/DIABLO.
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: Polyclonal
Isotype: Rabbit IgG

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Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications

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Description

The polyclonal antibody reacts with Smac/DIABLO; the antibody was raised against a peptide mapping to the carboxy terminus of human Smac/DIABLO. Second mitochondrial activator of caspases or Smac/DIABLO can promote apoptosis by relieving the inhibition of caspases by the IAPs (1&2). The other mitochondrial activator of apoptosis is cytochrome c (1). Smac/DIABLO export from the mitochondria into the cytosol can be triggered by cytotoxic drugs, DNA damage, and the ligation of FAS (CD95) death receptor (3). Once in the cytosol Smac/DIABLO eliminates the inhibitory effects of the IAPs through and amino terminal interaction with the IAP proteins, and thus allow the activation of caspases (2&4). The interaction of Smac/DIABLO?s amino terminus with the IAP proteins has been localized to their BIR domains, for XIAP the key domain is BIR3 (4&5).

Applications Reported

For research use only, not for diagnostic or therapeutic use. Purified anti-human Smac/Diablo poly has been reported for use in immunoblotting (WB).

Applications Tested

Purified anti-human Smac/Diablo poly has been tested by immunoblotting (WB). (1:200 starting dilution). It is recommended that this antibody be titrated for optimal performance in the assay of interest.

References

1. Du C., Fang M., Li Y., Li L., Wang X. 2000. Smac, a Mitochondrial Protein that promotes Cytochrome c-Dependent Caspase Activation by Eliminating IAP Inhibition. Cell 102:33-42.
2. Springs S.L., Diavolitsis V.M., Goodhouse J., Mclendon G.L. 2002. The kinetics of translocation of Smac/DIABLO from the mitochondria to the cytosol in HeLa cells. J Biol Chem
3. Adrain C., Creagh E.M., Martin S.J. 2001. Apoptosis-associated release of Smac/DIABLO from mitochondria requires active caspases and is blocked by Bcl-2. EMBO J 20(23):6627-6636.
4. Wu G., Chai J., Suber T.L., Wu J.W., Du C., Wang X., Shi Y. 2000. Structural basis of IAP recognition by Smac/DIABLO. Nature 408(6815):1008-1012.
5. Liu Z., Sun C., Olejniczak E.T., Meadows R.P., Betz S.F., Oost T., Herrmann J., Wu J.C., Fesik S.W. 2000. Structural basis for binding of Smac/DIABLO to the XIAP BIR3 domain. Nature 408(6815):1004-1008.

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