mouse/human PUMA (bbc3) antibody: purified antibody

Contents: Affinity Purified anti-mouse/human PUMA alpha/beta polyclonal
Catalog Number: 14-6740
Formulation: 200 µg/ml rabbit polyclonal IgG in PBS containing 0.1 % sodium azide and 0.2% gelatin.
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: Polyclonal
Isotype: Rabbit IgG

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
14-6740-63	10 ug	$30
14-6740-81	50 ug	$115

*International customers: Please contact your distributor for region specific pricing.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-6740	Purified anti-mouse/human PUMA alpha/beta polyclonal	N/A	N/A	WB

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Description

The polyclonal antibody reacts with mouse and human PUMA; the antibody was generated against a peptide mapping to an internal domain of human PUMA. The Bcl-2 family proteins can function to either suppress or promote cell death and are characterized by the presence of up to four conserved amino acid motifs, termed Bcl-2 homology (BH) domains (1). The BH3 function has been further revealed by an emerging group of BH3-only proteins, that includes Bik, Bad, Bid, Bim, Noxa, and the newest member PUMA or bbc3 (1&2). The PUMA gene is a target of p53 transcriptional activation and encodes two BH3 domain-containing proteins (PUMA-alpha and PUMA-beta) (3&4). The activities of PUMA-alpha and PUMA-beta are similar; they bind to Bcl-2, localize to the mitochondria to induce cytochrome c release, and activate extremely rapid apoptosis (3&4).

Applications Reported

For research use only, not for diagnostic or therapeutic use. Purified anti-mo/hu PUMA alpha/beta poly has been reported for use in immunoblotting (WB).

Applications Tested

Purified anti-mo/hu PUMA alpha/beta poly has been tested by immunoblotting (WB). (1:200 starting dilution). It is recommended that this antibody be titrated for optimal performance in the assay of interest.

References

1. Han J., Flemington C., Houghton A.B., Gu Z., Zambetti G.P., Lutz R.J., Zhu L., Chittenden T. 2001. Expression of bbc3, a pro-apoptotic BH3-only gene, is regulated by diverse cell death and survival signals. PNAS 98(20):11318-11323.
2. Schuler M., Green D.R. 2001. Mechanisms of p53-dependent apoptosis. Biochem Soc Trans 29(Pt6):684-688.
3. Nakano K., Vousden K.H. 2001. PUMA, a novel proapoptotic gene, is induced by p53. Mol Cell 7(3):683-694.
4. Yu J., Zhang L., Hwang P.M., Kinzler K.W., Vogelstein B. 2001. PUMA induces the rapid apoptosis of colorectal cancer cells. Mol Cell 7(3):673-682.

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