human TRAIL (CD253, Apo-2L) antibody: purified antibody

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Contents: Affinity Purified anti-human TRAIL (CD253) polyclonal
Catalog Number: 14-6737
Formulation: 200 µg/ml rabbit polyclonal IgG in PBS containing 0.1 % sodium azide and 0.2% gelatin.
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: Polyclonal
Isotype: Rabbit IgG

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
14-6737-63	10 ug	$30
14-6737-81	50 ug	$115

*International customers: Please contact your distributor for region specific pricing.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-6737	Purified anti-human TRAIL (CD253) polyclonal	N/A	N/A	WB

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The polyclonal antibody reacts with TRAIL; the antibody was raised against a peptide mapping to the carboxy terminus of human TRAIL. Several members of the growing TNF superfamily can regulate the elimination of immune cells by inducing apoptosis (1). TRAIL (TNF-related apoptosis-inducing ligand) or APO-2L is a member of the TNF superfamily that induces apoptosis by activating the death receptors DR4 and DR5 (2,3). TRAIL is attracting great interesting as an anti-cancer agent because it can induce apoptosis in a broad range of tumor cells, but only rarely in non-transformed cells, and independently of p53 status (2&4). For a time it was a it was unclear how TRAIL could induce apoptosis in tumor cells but not in non-transformed cells, while both of the cell types expressed the DR4 and DR5 death receptors. The discovery that DcR1 or TRID are mostly expressed on normal cells but not in most cancer cell lines helped to solve the puzzle, because these proteins act as decoys for the TRAIL protein (5).

Applications Reported

For research use only, not for diagnostic or therapeutic use. Purified anti-human TRAIL poly has been reported for use in immunoblotting (WB).

Applications Tested

Purified anti-human TRAIL poly has been tested by immunoblotting (WB). (1:200 starting dilution). It is recommended that this antibody be titrated for optimal performance in the assay of interest.

References

1. Pitti R. M., Marsters S.A., Siegfried R., Donahue C.J., Moore A., Ashkenazi A. 1996. Induction of Apoptosis by Apo-2 ligand, a New Member of the Tumor Necrosis Factor Cytokine Family. JBC 271(22):12687-12690.
2. Wajant H., Pfizenmaier K., Scheurich P. 2002. TNF-related apoptosis inducing ligand (TRAIL) and its receptors in tumor surveillance and cancer therapy. Apoptosis 7(5):449-459.
3. Phillips T.A., Ni J., Pan G., Ruben S.M., Wei Y., Pace J.L., Hunt J.S. 1999. TRAIL (Apo-2L) and TRAIL Receptors in Human Placentas: Implications for Immune Privilege. Journal of Imm 162:6053-6059.
4. 4. Nagane M., Huang H.J., Cavenee W.K. 2001. The potential of TRAIL for cancer chemotherapy. Apoptosis 6(3):191-197.
5. Gura T. 1997. How TRAIL Kills Cancer Cells, But not Normal Cells. Science 277(8):768.

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