mouse/rat/human c-Fos antibody: purified antibody

Contents: Affinity Purified anti-mouse/human/rat c-Fos polyclonal
Catalog Number: 14-6723
Formulation: 200 µg/ml rabbit polyclonal IgG in PBS containing 0.1 % sodium azide and 0.2% gelatin.
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: Polyclonal
Isotype: Rabbit IgG

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
14-6723-63	10 ug	$30
14-6723-81	50 ug	$115

*International customers: Please contact your distributor for region specific pricing.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-6723	Purified anti-mouse/human/rat c-Fos polyclonal	N/A	N/A	IHC IP WB

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The polyclonal antibody reacts with mouse, human, and rat c-Fos; the antibody was raised against a peptide mapping to the amino terminus of human c-Fos. The products of the proto-oncogenes c-Jun (c-Jun was previously known as the Fos-binding protein p39) and c-Fos are known to form a complex in the nucleus (2, 6). AP-1 (activating protein-1) is a collective term referring to these dimeric transcription factors composed of Jun, Fos or ATF (activating transcription factor) subunits that bind to a common DNA site, the AP-1-binding site (1). There is evidence that AP-1 proteins, mostly those that belong to the Jun group, control cell life and death through their ability to regulate the expression and function of cell cycle regulators such as Cyclin D1, p53, p21 (cip1/waf1), p19 (ARF) and p16 (3). The Fos and Jun proto-oncogenes expression is induced transiently by a great variety of extracellular stimuli associated with mitogenesis, differentiation processes or depolarization of neurons (5). Studies have established a unique role for Fos in determining the differentiation and activity of progenitors of the osteoclast lineage (4).

Applications Reported

For research use only, not for diagnostic or therapeutic use. Purified anti-mo/hu/rat c-Fos poly has been reported for use in immunoprecipitation, immunoblotting (WB), and immunohistochemical staining.

Applications Tested

Purified anti-mo/hu/rat c-Fos poly has been tested by immunoblotting (WB). (1:200 starting dilution). It is recommended that this antibody be titrated for optimal performance in the assay of interest.

References

1. Karin M, Liu Zg, Zandi E. AP-1 function and regulation.Curr Opin Cell Biol. 1997 Apr;9(2):240-6.
2. Bos TJ, Rauscher FJ 3rd, Curran T, Vogt PK. The carboxy terminus of the viral Jun oncoprotein is required for complex formation with the cellular Fos protein. Oncogene. 1989 Feb;4(2):123-6.
3. Shaulian E, Karin M. AP-1 in cell proliferation and survival. Oncogene. 2001 Apr 30;20(19):2390-400.
4. Liebermann DA, Gregory B, Hoffman B. AP-1 (Fos/Jun) transcription factors in hematopoietic differentiation and apoptosis. Int J Oncol. 1998 Mar;12(3):685-700
5. Curran T. Fos and Jun: oncogenic transcription factors.Tohoku J Exp Med. 1992 Oct;168(2):169-74.
6. Rauscher FJ 3rd, Voulalas PJ, Franza BR Jr, Curran T. Fos and Jun bind cooperatively to the AP-1 site: reconstitution in vitro. Genes Dev. 1988 Dec;2(12B):1687-99.

Related Products

Cat. 66-P723 Blocking Peptides for anti-mouse/human/rat c-Fos polyclonal