mouse/rat/human Cdk2 antibody: purified antibody

Contents: Affinity Purified anti-mouse/human/rat Cdk2 polyclonal
Catalog Number: 14-6709
Formulation: 200 µg/ml rabbit polyclonal IgG in PBS containing 0.1% sodium azide and 0.2% gelatin.
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: Polyclonal
Isotype: Rabbit IgG

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
14-6709-63	10 ug	$30
14-6709-81	50 ug	$115

*International customers: Please contact your distributor for region specific pricing.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-6709	Purified anti-mouse/human/rat Cdk2 polyclonal	N/A	N/A	IHC IP WB

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Description

The polyclonal antibody reacts with mouse, human, and rat Cdk2; the antibody was raised against a peptide mapping to the carboxy terminus of human Cdk2. Cyclin dependent kinases are key regulators of the progression of the cell cycle. Early in the cell cycle Cdk2, Cdk4, Cdk6 and their associated cyclins regulate the G1 to S phase transition (1, 2). Cdk2 plays a key role in the G1/S and S/G2 transitions through its associations with cyclin D1, cyclin D2, cyclin D3, cyclin E and cyclin A. Cdk4 also forms complexes with the D type cyclins, and is thought to regulate cell growth through the G1 phase of the cell cycle (3-6). The late stages of the cell cycle are regulated by another cyclin dependent kinase, Cdc2 p34. This kinase exists as a complex with both cyclin A and cyclin B. The best characterized of these associations is the Cdc2 p34-cyclin B complex that is required for the G2 to M phase transition (7,8).

Applications Reported

For research use only, not for diagnostic or therapeutic use. Purified anti-mo/hu/rat Cdk2 poly has been reported for use in immunoprecipitation, immunoblotting (WB), and immunohistochemical staining.

Applications Tested

Purified anti-mo/hu/rat Cdk2 poly has been tested by immunoblotting (WB) (1:400 starting dilution). HeLa, HL-60 and NIH/3T3 whole cell lysates can be used as positive controls.

References

1. Ekholm SV, Reed SI. 2000. Regulation of G(1) cyclin-dependent kinases in the mammalian cell cycle. Curr Opin Cell Biology 12(6): 676-684
2. Morisaki H, Ando A, Nagata Y, Pereira-Smith O, Smith JR, Ikeda K, Nakanishi M. 1999. Complex mechanisms underlying impaired activation of Cdk4 and Cdk2 in replicative senescence: roles of p16, p21, and cyclin D1. Exp Cell Research 253(2): 503-510.
3. Tong W, Pollard JW. 1999. Progesterone inhibits estrogen-induced cyclin D1 and cdk4 nuclear translocation, cyclin E- and cyclin A-cdk2 kinase activation, and cell proliferation in uterine epithelial cells in mice. Mol Cell Biology 19(3): 2251-64.
4. Sweeney KJ, Sarcevic B, Sutherland RL, Musgrove EA. 1997. Cyclin D2 activates Cdk2 in preference to Cdk4 in human breast epithelial cells. Oncogene 14(11): 1329-1340.
5. Chu CY, Lim RW. 2000. Involvement of p27(kip1) and cyclin D3 in the regulation of cdk2 activity during skeletal muscle differentiation. Biochimica Biophysica Acta 1497(2): 175-185.
6. Kishimoto T, Okumura E. In vivo regulation of the entry into M-phase: initial activation and nuclear translocation of cyclin B/Cdc2. 1997. Prog Cell Cycle Res 3:241-249.
7. Morla AO, Draetta G, Beach D, Wang JY. 1989. Reversible tyrosine phosphorylation of cdc2: dephosphorylation accompanies activation during entry into mitosis. Cell 58(1): 193-203.
8. O?Connor PM, Ferris DK, Pagano M, Draetta G, Pines J, Hunter T, Longo DL, Kohn KW. 1993. G2 delay induced by nitrogen mustard in human cells affects cyclin A/cdk2 and cyclin B1/cdc2-kinase complexes differently. J Biological Chemistry 268(11): 8298-8308.

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