Product Information

Contents: Affinity Purified anti-human RANKL (CD254, RANK-L, TRANCE, OPGL)
Catalog Number: 14-6619
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: MIH24
Isotype: Mouse IgG2b, κ

Staining of non-transfected (left) and human RANKL transfected cells (right) with 0.25 μg of Purified Mouse IgG2b Iso Cntrl (cat. 14-4732) (open histogram) or 0.5 μg of Purified MIH24 (colored histogram) followed by FITC Anti-Mouse IgG (cat. 11-4011). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-6619	Purified anti-human RANKL (CD254, RANK-L, TRANCE, OPGL)	N/A	N/A	ELISA det FC
13-6619	Biotin anti-human RANKL (CD254, RANK-L, TRANCE, OPGL)	N/A	N/A	ELISA det FC
12-6619	Phycoerythrin (PE) anti-human RANKL (CD254, RANK-L, TRANCE, OPGL)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The MIH24 monoclonal antibody reacts with human TRANCE (TNF-related activation-induced cytokine receptor) also known as RANKL (receptor activator of NF-κB ligand) and OPGL (Osteoprotegerin Ligand). TRANCE is a type II membrane protein with predicted molecular weight of 35 kDa. Its extracellular domain is most closely related to TRAIL, FasL and TNF. TRANCE mRNA is upregulated by TCR stimulation. TRANCE-deficient mouse lacks osteoclasts. In addition, TRANCE has been shown to be involved in the interactions between T-dendritic and T-B cells. It was also found to be critical in osteoclast differentiation. The TRANCE-RANK interaction regulates the expression of the anti-apoptotic molecule Bcl-x_L.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The MIH24 antibody has been reported for use in flow cytometric analysis, and ELISA.

Applications Tested

The MIH24 antibody has been tested by flow cytometric analysis of human RANKL transfected cells. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Kanamaru F, Iwai H, Ikeda T, Nakajima A, Ishikawa I, Azuma M. 2004. Expression of membrane-bound and soluble receptor activator of NF-kappaB ligand (RANKL) in human T cells. Immunol Lett. 94(3):239-46.

Kanamaru F, Iwai H, et al. 2004. Expression of membrane-bound and soluble receptor activator of NF-kappaB ligand (RANKL) in human T cells. Immunol Lett. 94(3):239-46. (MIH24, RANKL in Th1 vs Th2, PubMed)

Related Products

Cat. 11-4011  FITC Anti-Mouse IgG
Cat. 13-4013  Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4732  Affinity Purified Mouse IgG2b Isotype Control
Cat. 14-6617  Purified anti-human RANKL (CD254, RANK-L, TRANCE, OPGL) (clone MIH23)