Product Information

Contents: Affinity Purified anti-human RANKL (CD254, RANK-L, TRANCE, OPGL)
Catalog Number: 14-6617
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
500 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: MIH23
Isotype: Mouse IgM

Staining of human RANKL transfected cells with 0.25 μg of Purified Mouse IgM Iso Cntrl (cat. 14-4752) (open histogram) or 0.125 μg of Purified MIH23 (colored histogram) followed by FITC Anti-Mouse IgG (cat. 11-4011). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-6617	Purified anti-human RANKL (CD254, RANK-L, TRANCE, OPGL)	N/A	N/A	ELISA cap FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The MIH23 monoclonal antibody reacts with human TRANCE (TNF-related activation-induced cytokine receptor) also known as RANKL (receptor activator of NF-κB ligand) and OPGL (Osteoprotegerin Ligand). TRANCE is a type II membrane protein with predicted molecular weight of 35 kDa. Its extracellular domain is most closely related to TRAIL, FasL and TNF. TRANCE mRNA is upregulated by TCR stimulation. TRANCE-deficient mouse lacks osteoclasts. In addition, TRANCE has been shown to be involved in the interactions between T-dendritic and T-B cells. It was also found to be critical in osteoclast differentiation. The TRANCE-RANK interaction regulates the expression of the anti-apoptotic molecule Bcl-x_L.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The MIH23 antibody has been reported for use in flow cytometric analysis, and ELISA.

Applications Tested

The MIH23 antibody has been tested ELISA. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Kanamaru F, Iwai H, Ikeda T, Nakajima A, Ishikawa I, Azuma M. 2004. Expression of membrane-bound and soluble receptor activator of NF-kappaB ligand (RANKL) in human T cells. Immunol Lett. 94(3):239-46.

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