Product Information

Contents: Affinity Purified anti-mouse RANK (CD265, Receptor Activator of NF-κB)
Catalog Number: 14-6612
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: R12-31
Isotype: Rat IgG2a, κ

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-6612	Purified anti-mouse RANK (CD265, Receptor Activator of NF-kB)	N/A	N/A	FC
13-6612	Biotin anti-mouse RANK (CD265, Receptor Activator of NF-kB)	N/A	N/A	FC
12-6612	Phycoerythrin (PE) anti-mouse RANK (CD265, Receptor Activator of NF-kB)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The R12-31 monoclonal antibody reacts with mouse RANK (Receptor activator of NF-κB), a recently cloned member of the TNFR superfamily with no significant homology to other members of this family. RANK ligand (RANKL/TRANCE/ OPGL) binds to RANK on dendritic cells, upregulates the expression of anti-apoptotic protein BcL-X_L suggesting a role in dendritic cell survival. The cytoplasmic domain of RANK interacts with TRAF2, TRAF5 and TRAF6. Overexpression of RANK activates NF-κB and c-Jun-terminal kinase (JNK) pathways. Recent studies have shown that RANK interaction with TRAF6 activates NF-κB, whereas JNK activation is mediated through binding of RANK to TRAF2.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The R12-31 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The R12-31 antibody has been tested by flow cytometric analysis of mouse RANK transfected cells. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

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