14-6409

Contents: Affinity Purified anti-mouse/human/rat Smac (DIABLO) (CT) polyclonal
Catalog Number: 14-6409
Storage Conditions: It is supplied as immunoaffinity chromatography purified IgG, in PBS containing 0.02% sodium azide. Store at 4°C, stable for one year.
Clone: Polyclonal
Isotype: Rabbit IgG

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Description

The polyclonal antibody reacts with human, mouse, and rat Smac/DIABLO; the antibody was raised against a peptide (eeraeseqeaylred) corresponding to amino acids 225 to 239 of human Smac/DIABLO (1). The inhibitor of apoptosis proteins (IAPs) regulate programmed cell death by inhibiting members of the caspase family of enzymes. A novel mammalian protein that binds to IAPs and neutralizes the inhibitory effect of IAPs on caspases was recently identified and designated Smac/DIABLO (1,2). Smac/DIABLO is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates cytochrome c/Apaf-1/capase-9 pathway. Analysis of the structural basis of Smac/DIABLO reveals that the N-terminal amino acids are required for binding of Smac/DIABLO to IAPs and activation of caspases (3-6). Smac/DIABLO is expressed in a variety of human and mouse tissues (1,2).

Applications Reported

For research use only, not for diagnostic or therapeutic use. This polyclonal antibody has been reported for use in immunoblotting (WB).

Applications Tested

This polyclonal antibody can be used for detection of Smac/DIABLO by Western blot at 0.5 to 1 µg/ml. Human heart tissue lysate can be used as positive control and a 25 kDa band can be detected. It is human, mouse, and rat reactive.

References

1. Du C, Fang M, Li Y, Li L, Wang X. Smac, a mitochondrial protein that promotes cytochrome c-dependent caspase activation by eliminating IAP inhibition. Cell. 2000;102(1):33-42.
2. Verhagen AM, Ekert PG, Pakusch M, Silke J, Connolly LM, Reid GE, Moritz RL, Simpson RJ, Vaux DL. Identification of DIABLO, a mammalian protein that promotes apoptosis by binding to and antagonizing IAP proteins. Cell. 2000;102(1):43-53.
3. Srinivasula SM, Datta P, Fan XJ, Fernandes-Alnemri T, Huang Z, Alnemri ES. Molecular Determinants of the Caspase-promoting Activity of Smac/DIABLO and Its Role in the Death Receptor Pathway. J Biol Chem. 2000;275(46):36152-36157.
4. Chai J, Du C, Wu JW, Kyin S, Wang X, Shi Y. Structural and biochemical basis of apoptotic activation by Smac/DIABLO. Nature. 2000;406(6798):855-62.
5. Liu Z, Sun C, Olejniczak ET, Meadows RP, Betz SF, Oost T, Herrmann J, Wu JC, Fesik SW. Structural basis for binding of Smac/DIABLO to the XIAP BIR3 domain. Nature. 2000;408(6815):1004-8.
6. Wu G, Chai J, Suber TL, Wu JW, Du C, Wang X, Shi Y. Structural basis of IAP recognition by Smac/DIABLO. Nature. 2000;408(6815):1008-12.

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