Contents: Affinity Purified anti-human Acinus (NP) polyclonal
Catalog Number: 14-6307
Storage Conditions: It is supplied as immunoaffinity chromatography purified IgG, in PBS containing 0.02% sodium azide. Store at 4°C, stable for one year.
Clone: Polyclonal
Isotype: Rabbit IgG
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Description
The rabbit anti-IKKa polyclonal antibody reacts with human Acinus; the antibody was raised against a peptide (ddpvrtaqvpspprgk) corresponding to amino acids 994 to 1009 of human AcinusL, 267 to 282 of human AcinusS’, or 236 to 251 of human AcinusS, which are identical to those of mouse Acinus (2). The selected antigenic sequence is located near the N-terminus of active peptide p17. Chromatin condensation and nuclear fragmentation (CCNF) is the hallmark of apoptosis. CCNF is triggered by the activation of members of caspase family, caspase activated DNase (CAD/DFF40), and several novel proteins including AIF and CIDE (1). A new inducer of chromatin condensation was recently identified and designated Acinus (for apoptotic chromatin condensation inducer in the nucleus). Acinus is cleaved by caspase-3 and an additional unknown protease generating a small active peptide p17, which causes chromatin condensation in vitro when it is added to purified nuclei. Acinus also induces apoptotic chromatin condensation in cells. Acinus is ubiquitously expressed. Three different spliced forms of Acinus have been identified in human and mouse and designated AcinusL, AcinusS and AcinusS’ (2).
Applications Reported
For research use only, not for diagnostic or therapeutic use. This polyclonal antibody has been reported for use in immunoblotting (WB).
Applications Tested
This polyclonal antibody can be used for detection of Acinus by Western blot at 0.5 to 1 µg/ml. K562 cell lysate can be used as positive control and an approximate 220 kDa band can be detected.
References
1. Zamzami N, Kroemer G. Condensed matter in cell death. Nature 1999 ;401:127-8.
2. Sahara S, Aoto M, Eguchi Y, Imamoto N, Yoneda Y, Tsujimoto Y. Acinus is a caspase-3-activated protein required for apoptotic chromatin condensation. Nature 1999 401:168-73.
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