Contents: Anti-mouse/rat RIP3 polyclonal
Catalog Number: 14-6283
Storage Conditions: This product is supplied as purified rabbit IgG, in PBS containing 0.02% NaN3. Store at 4°C, stable for one year.
Clone: Polyclonal
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Description
This rabbit polyclonal antibody was raised against a peptide (aqfgrgrgwqpfhk) corresponding to amino acids 473 to 486 of murine RIP3. Certain serine/threonine protein kinases, such as ASK1, RIP, DAP, and ZIP kinases, are mediators of apoptosis. Receptor interacting proteins including RIP and RIP2/RICK mediate apoptosis induced by TNFR1 and Fas, two prototype members in the death receptor family. A novel member in the RIP kinase family was recently identified and designated RIP3. RIP3 contains N-terminal kinase domain but, unlike RIP or RIP2, lacks the C-terminal death or CARD domain. RIP3 binds to RIP and TNFR1, mediates TNFR1 induced apoptosis, and attenuates RIP and TNFR1 induced NF-κB activation. Overexpression of RIP3 induces apoptosis and NF-κB activation. The messenger RNA of RIP3 is expressed in a subset of adult tissues.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This polyclonal antibody has been reported for use in immunoblotting (WB).
Applications Tested
This polyclonal antibody has been tested by immunoblotting (WB) (0.5-1 μg/ml). 3T3 cell lysate can be used as positive control and an approximately 57 kDa band can be detected. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Yu PW, Huang BC, Shen M, Quast J, Chan E, Xu X, Nolan GP, Payan DG, Luo Y. Identification of RIP3, a RIP-like kinase that activates apoptosis and NFkappaB. Curr Biol. 1999;9(10):539-42.
Sun X, Lee J, Navas T, Baldwin DT, Stewart TA, Dixit VM. RIP3, a novel apoptosis-inducing kinase. J Biol Chem. 1999;274(24):16871-5.
Pazdernik NJ, Donner DB, Goebl MG, Harrington MA. Mouse receptor interacting protein 3 does not contain a caspase-recruiting or a death domain but induces apoptosis and activates NF-kappaB. Mol Cell Bio. 1999; 19(10):6500-8.
